氢离子/离子反向转运作为卡尔斯伯酵母液泡膜转运系统的主要机制。
H+/ion antiport as the principal mechanism of transport systems in the vacuolar membrane of the yeast Saccharomyces carlsbergensis.
作者信息
Okorokov L A, Kulakovskaya T V, Lichko L P, Polorotova E V
出版信息
FEBS Lett. 1985 Nov 18;192(2):303-6. doi: 10.1016/0014-5793(85)80130-7.
The secondary transport systems of the yeast vacuolar membrane have been investigated by the method of radioactive isotopes [( 14C]arginine); activation of H+-ATPase by cations (Cat+), when the enzyme is under H+ control and measurement of changes in the proton gradient (delta pH) and membrane potential (Em) due to the supposed substrates of the transporters. The main mechanism of cation transport across the yeast tonoplast is probably H+/Cat+ antiport. The apparent Km of antiporters for Ca2+, Mg2+, Mn2+, Zn2+ and Pi are 0.06, 0.3, 0.8, 0.055-0.17 and 1.5 mM, respectively.
利用放射性同位素（[¹⁴C]精氨酸）法对酵母液泡膜的次级转运系统进行了研究；当酶处于H⁺控制下时，通过阳离子（Cat⁺）对H⁺-ATP酶的激活作用，以及测量由于转运体假定底物导致的质子梯度（ΔpH）和膜电位（Em）的变化。阳离子跨酵母液泡膜转运的主要机制可能是H⁺/Cat⁺反向转运。反向转运体对Ca²⁺、Mg²⁺、Mn²⁺、Zn²⁺和Pi的表观Km分别为0.06、0.3、0.8、0.055 - 0.17和1.5 mM。
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