UMR Bioger, INRA, AgroParisTech, Université Paris-Saclay, Avenue Lucien Brétignières, Thiverval-Grignon, F-78850, France.
INRA, UMR 1349 IGEPP, BP35327, Le Rheu Cedex F-35653, France.
Mol Plant Pathol. 2018 Apr;19(4):1012-1016. doi: 10.1111/mpp.12574. Epub 2017 Sep 20.
Leptosphaeria maculans, the causal agent of blackleg disease, interacts with Brassica napus (oilseed rape, canola) and other Brassica hosts in a gene-for-gene manner. The avirulence gene AvrLmJ1 has been cloned previously and shown to interact with an unidentified Brassica juncea resistance gene. In this study, we show that the AvrLmJ1 gene maps to the same position as the AvrLm5 locus. Furthermore, isolates complemented with the AvrLmJ1 locus confer avirulence towards B. juncea genotypes harbouring Rlm5. These findings demonstrate that AvrLmJ1 is AvrLm5 and highlight the need for shared resources to characterize accurately avirulence and/or resistance genes.
导致黑胫病的病原菌李属坏死斑点菌(Leptosphaeria maculans)以基因对基因的方式与油菜( Brassica napus ,即菜籽、芥花油)和其他芸薹属作物互作。先前已克隆出无毒基因 AvrLmJ1,其被证实与一个未鉴定的芸薹属芥菜抗性基因互作。本研究表明,AvrLmJ1 基因与 AvrLm5 基因座定位于同一位置。此外,含有 AvrLmJ1 基因座的分离物对含有 Rlm5 的芸薹属芥菜基因型表现无毒。这些发现表明,AvrLmJ1 是 AvrLm5,突出了准确鉴定无毒和/或抗性基因所需的共享资源。