Ruminant Diseases and Immunology Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, PO Box 70, 1920 Dayton Avenue, Ames, IA, 50010, USA.
Ruminant Diseases and Immunology Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, PO Box 70, 1920 Dayton Avenue, Ames, IA, 50010, USA.
Virus Res. 2017 Jun 15;238:198-203. doi: 10.1016/j.virusres.2017.06.020. Epub 2017 Jun 27.
A novel adenovirus, CeAdV1, was isolated from buffy coat and nasal swab samples collected from two captive white-tailed deer (Odocoileus virginianus) fawns. The isolation was an incidental finding in the course of screening animals for use in a research study on an unrelated pathogen. In the screening process, virus isolation was performed on both nasal swabs and buffy coat samples and cytopathic effect was observed. Electron microscopy revealed viral particles with the shape and morphology of an adenovirus. Next generation sequencing followed by phylogenetic analysis classified this virus to the Mastadenovirus genus. Its sequence was genetically distinct from all other recognized species in this genus, with only 76% sequence identity to its closest genetic match, bovine adenovirus 3 (BAdV3). The virus could be propagated in bovine derived cells but grew to a higher titer in cervid derived cells. Inoculation of white-tailed deer fawns with the isolated virus resulted in pyrexia, depletion of thymus tissue and mild respiratory disease. Comparative serology performed using convalescent sera revealed distinct antigenic differences between the novel cervid adenovirus and BAdV3. A retrospective serological survey of the captive deer herd indicated that this virus had been circulating in the herd for at least 14 years with no report of clinical disease. A survey of serum collected from free ranging mule deer residing in Nevada revealed high serum titers against this novel adenovirus.
一种新型腺病毒，即 CeAdV1，从两只圈养白尾鹿（Odocoileus virginianus）幼鹿的血涂片和鼻腔拭子样本中分离得到。该分离是在对用于与无关病原体相关的研究的动物进行筛选过程中的偶然发现。在筛选过程中，对鼻腔拭子和血涂片样本进行了病毒分离，并观察到细胞病变效应。电子显微镜显示，病毒粒子具有腺病毒的形状和形态。随后的下一代测序和系统发育分析将该病毒归类为 Mastadenovirus 属。其序列与该属中所有其他已识别的物种在遗传上均不同，与最接近的遗传匹配物，即牛腺病毒 3（BAdV3）的序列同一性仅为 76%。该病毒可在牛源细胞中增殖，但在鹿源细胞中生长到更高的滴度。用分离的病毒接种白尾鹿幼鹿会导致发热、胸腺组织耗竭和轻度呼吸道疾病。使用恢复期血清进行的比较血清学研究表明，这种新型鹿腺病毒与 BAdV3 之间存在明显的抗原差异。对圈养鹿群的回顾性血清学调查表明，该病毒至少在该鹿群中流行了 14 年，没有报告临床疾病。对居住在内华达州的自由放养骡鹿采集的血清进行的调查显示，针对这种新型腺病毒的血清抗体效价很高。
