Wang Ling, Wu Yaobin, Hu Tianli, Guo Baolin, Ma Peter X
Frontier Institute of Science and Technology, and State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, China.
Frontier Institute of Science and Technology, and State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, China.
Acta Biomater. 2017 Sep 1;59:68-81. doi: 10.1016/j.actbio.2017.06.036. Epub 2017 Jun 27.
Mimicking the nanofibrous structure similar to extracellular matrix and conductivity for electrical propagation of native myocardium would be highly beneficial for cardiac tissue engineering and cardiomyocytes-based bioactuators. Herein, we developed conductive nanofibrous sheets with electrical conductivity and nanofibrous structure composed of poly(l-lactic acid) (PLA) blending with polyaniline (PANI) for cardiac tissue engineering and cardiomyocytes-based 3D bioactuators. Incorporating of varying contents of PANI from 0wt% to 3wt% into the PLA polymer, the electrospun nanofibrous sheets showed enhanced conductivity while maintaining the same fiber diameter. These PLA/PANI conductive nanofibrous sheets exhibited good cell viability and promoting effect on differentiation of H9c2 cardiomyoblasts in terms of maturation index and fusion index. Moreover, PLA/PANI nanofibrous sheets enhanced the cell-cell interaction, maturation and spontaneous beating of primary cardiomyocytes. Furthermore, the cardiomyocytes-laden PLA/PANI conductive nanofibrous sheets can form 3D bioactuators with tubular and folding shapes, and spontaneously beat with much higher frequency and displacement than that on cardiomyocytes-laden PLA nanofibrous sheets. Therefore, these PLA/PANI conductive nanofibrous sheets with conductivity and extracellular matrix like nanostructure demonstrated promising potential in cardiac tissue engineering and cardiomyocytes-based 3D bioactuators.
Cardiomyocytes-based bioactuators have been paid more attention due to their spontaneous motion by integrating cardiomyocytes into polymer structures, but developing suitable scaffolds for bioactuators remains challenging. Electrospun nanofibrous scaffolds have been widely used in cardiac tissue engineering because they can mimic the extracellular matrix of myocardium. Developing conductive nanofibrous scaffolds by electrospinning would be beneficial for cardiomyocytes-based bioactuators, but such scaffolds have been rarely reported. This work presented a conductive nanofibrous sheet based on polylactide and polyaniline via electrospinning with tunable conductivity. These conductive nanofibrous sheets performed the ability to enhance cardiomyocytes maturation and spontaneous beating, and further formed cardiomyocytes-based 3D bioactuators with tubular and folding shapes, which indicated their great potential in cardiac tissue engineering and bioactuators applications.
模仿类似于细胞外基质的纳米纤维结构以及具备天然心肌电传导所需的导电性，对于心脏组织工程和基于心肌细胞的生物致动器将大有裨益。在此，我们开发了具有导电性和纳米纤维结构的导电纳米纤维片，其由聚（L-乳酸）（PLA）与聚苯胺（PANI）共混而成，用于心脏组织工程和基于心肌细胞的三维生物致动器。将不同含量（0wt%至3wt%）的聚苯胺掺入聚乳酸聚合物中，电纺纳米纤维片在保持相同纤维直径的同时，导电性增强。这些聚乳酸/聚苯胺导电纳米纤维片表现出良好的细胞活力，并在成熟指数和融合指数方面对H9c2心肌母细胞的分化具有促进作用。此外，聚乳酸/聚苯胺纳米纤维片增强了原代心肌细胞的细胞间相互作用、成熟度和自发搏动。此外，负载心肌细胞的聚乳酸/聚苯胺导电纳米纤维片可形成管状和折叠形状的三维生物致动器，并且与负载心肌细胞的聚乳酸纳米纤维片相比，能以更高的频率和位移自发搏动。因此，这些具有导电性和类似细胞外基质纳米结构的聚乳酸/聚苯胺导电纳米纤维片在心脏组织工程和基于心肌细胞的三维生物致动器中显示出巨大的潜力。
基于心肌细胞的生物致动器因将心肌细胞整合到聚合物结构中实现自发运动而受到更多关注，但为生物致动器开发合适的支架仍然具有挑战性。电纺纳米纤维支架因其可模仿心肌的细胞外基质而被广泛应用于心脏组织工程。通过电纺开发导电纳米纤维支架对基于心肌细胞的生物致动器有益，但此类支架鲜有报道。这项工作通过电纺呈现了一种基于聚乳酸和聚苯胺的具有可调导电性的导电纳米纤维片。这些导电纳米纤维片具备增强心肌细胞成熟度和自发搏动的能力，并进一步形成了管状和折叠形状的基于心肌细胞的三维生物致动器，这表明它们在心脏组织工程和生物致动器应用中具有巨大潜力。
