Pérez-Moreno Tonantzi, D'Urso Claudia, Trejo Gabriel, Contreras-Martínez Maria V, Lozano Omar, García-Rivas Gerardo J, Arriaga Luis G, Luna-Barcenas Gabriel, Ledesma-García Janet
Facultad de Ingenieía, División de Investigación y Posgrado, Universidad Autónoma de Querérato, Santiago de Querétaro 76010, Querétaro, Mexico.
Consiglio Nazionale delle Ricerche Istituto di Tecnologie Avanzate per l'Energia "Nicola Giordano", Via S. Lucia Sopra Contesse, 5, 98126 Messina, Italy.
Membranes (Basel). 2025 Jun 11;15(6):177. doi: 10.3390/membranes15060177.
In this study, membranes of collagen-chitosan (C-Ch) in combination with conductive polymers (CPs) such as polyaniline (Pani) and polypyrrole (Ppy) were obtained by electrospinning using non-toxic solvents such as PBS and ethanol. The change in the morphology after swelling was observed by SEM, while an FTIR analysis showed specific interactions between C-Ch and CP. Mechanical tests showed that C-Ch/Ppy exhibited more elastic behavior and a better stress distribution compared to C-Ch/Pani. The diffusion of Na and Ca ions through the membranes was evaluated and showed a greater resistance for Ca in both membrane types. Preliminary biocompatibility testing with H9C2 cells showed a successful cell adhesion to the membranes. These results emphasize the potential of C-Ch/Pani composites for electrically active scaffolds and of C-Ch/PPy composites for applications in mechanically dynamic tissue-specific regeneration.
在本研究中，通过使用无毒溶剂如磷酸盐缓冲盐水（PBS）和乙醇进行静电纺丝，获得了胶原蛋白 - 壳聚糖（C-Ch）与导电聚合物（CPs）如聚苯胺（Pani）和聚吡咯（Ppy）结合的膜。通过扫描电子显微镜（SEM）观察溶胀后形态的变化，而傅里叶变换红外光谱（FTIR）分析显示了C-Ch和CP之间的特定相互作用。力学测试表明，与C-Ch/Pani相比，C-Ch/Ppy表现出更具弹性的行为和更好的应力分布。评估了Na和Ca离子通过膜的扩散情况，结果表明两种膜类型对Ca的阻力都更大。用H9C2细胞进行的初步生物相容性测试表明细胞成功粘附在膜上。这些结果强调了C-Ch/Pani复合材料在电活性支架方面的潜力以及C-Ch/PPy复合材料在机械动态组织特异性再生应用方面的潜力。
