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Two-dimensional spatiotemporal focusing of femtosecond pulses and its applications in microscopy.飞秒脉冲的二维时空聚焦及其在显微镜学中的应用。
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65-fs Yb-doped fiber laser system with gain-narrowing compensation.具有增益窄化补偿的65飞秒掺镱光纤激光系统。
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Pre-chirping management of a self-similar Yb-fiber amplifier towards 80 W average power with sub-40 fs pulse generation.面向 80 W 平均功率及亚 40 fs 脉冲产生的自相似 Yb 光纤放大器的预啁啾管理
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Measurements of multiphoton action cross sections for multiphoton microscopy.多光子显微镜的多光子作用截面测量
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three-photon microscopy of subcortical structures within an intact mouse brain.完整小鼠脑内皮层下结构的三光子显微镜检查
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Enhancement of lateral resolution and optical sectioning capability of two-photon fluorescence microscopy by combining temporal-focusing with structured illumination.通过将时间聚焦与结构照明相结合提高双光子荧光显微镜的横向分辨率和光学切片能力。
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Improvement of axial resolution and contrast in temporally focused widefield two-photon microscopy with structured light illumination.通过结构光照明在时间聚焦宽场双光子显微镜中提高轴向分辨率和对比度。
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Spatiotemporal focusing-based widefield multiphoton microscopy for fast optical sectioning.基于时空聚焦的宽场多光子显微镜用于快速光学切片
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Wide-field multiphoton imaging of cellular dynamics in thick tissue by temporal focusing and patterned illumination.通过时间聚焦和图案照明对厚组织中的细胞动力学进行宽场多光子成像。
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Ultrafast widefield optical sectioning microscopy by multifocal temporal focusing.基于多焦点时间聚焦的超快宽视场光学切片显微镜术
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使用具有92飞秒镱光纤啁啾脉冲放大器的三光子激发荧光的时间聚焦显微镜。

Temporal focusing microscopy using three-photon excitation fluorescence with a 92-fs Yb-fiber chirped pulse amplifier.

作者信息

Toda Keisuke, Isobe Keisuke, Namiki Kana, Kawano Hiroyuki, Miyawaki Atsushi, Midorikawa Katsumi

机构信息

RIKEN Center for Advanced Photonics, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.

Graduate School of Science and Engineering, Saitama University, 255 Shimo-Okubo, Sakura, Saitama 338-8570, Japan.

出版信息

Biomed Opt Express. 2017 May 1;8(6):2796-2806. doi: 10.1364/BOE.8.002796. eCollection 2017 Jun 1.

DOI:10.1364/BOE.8.002796
PMID:28663907
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5480430/
Abstract

Temporal focusing (TF) microscopy is a wide-field two-photon excitation fluorescence (2PEF) microscopy technique, the optical sectioning capability of which is lower than that of point-scanning 2PEF microscopy. Here we demonstrate TF microscopy using three-photon excitation fluorescence (3PEF), which enhances the optical sectioning capability. As an excitation light source for the 3PEF, we developed an Yb-fiber chirped pulse amplifier, which produces 92-fs 9.0-μJ 1060-nm pulses at a repetition rate of 200 kHz. The optical sectioning capability was improved by a factor of 1.3 compared with that of 2PEF-TF microscopy. We also demonstrate dual-color imaging with both 2PEF and 3PEF.

摘要

时间聚焦（TF）显微镜是一种宽场双光子激发荧光（2PEF）显微镜技术，其光学切片能力低于点扫描2PEF显微镜。在此，我们展示了使用三光子激发荧光（3PEF）的TF显微镜，其增强了光学切片能力。作为3PEF的激发光源，我们开发了一种镱光纤啁啾脉冲放大器，它以200 kHz的重复频率产生92飞秒、9.0微焦、1060纳米的脉冲。与2PEF-TF显微镜相比，光学切片能力提高了1.3倍。我们还展示了2PEF和3PEF的双色成像。