使用三光子激发荧光的干涉时间聚焦显微镜。
Interferometric temporal focusing microscopy using three-photon excitation fluorescence.
作者信息
Toda Keisuke, Isobe Keisuke, Namiki Kana, Kawano Hiroyuki, Miyawaki Atsushi, Midorikawa Katsumi
机构信息
RIKEN Center for Advanced Photonics, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
Graduate School of Science and Engineering, Saitama University, 255 Shimo-Okubo, Sakura, Saitama 338-8570, Japan.
出版信息
Biomed Opt Express. 2018 Mar 6;9(4):1510-1519. doi: 10.1364/BOE.9.001510. eCollection 2018 Apr 1.
Abstract
Super-resolution microscopy has become a powerful tool for biological research. However, its spatial resolution and imaging depth are limited, largely due to background light. Interferometric temporal focusing (ITF) microscopy, which combines structured illumination microscopy and three-photon excitation fluorescence microscopy, can overcome these limitations. Here, we demonstrate ITF microscopy using three-photon excitation fluorescence, which has a spatial resolution of 106 nm at an imaging depth of 100 µm with an excitation wavelength of 1060 nm.
摘要
超分辨率显微镜已成为生物学研究的强大工具。然而，其空间分辨率和成像深度受到限制，这主要归因于背景光。干涉时间聚焦（ITF）显微镜结合了结构照明显微镜和三光子激发荧光显微镜，能够克服这些限制。在此，我们展示了使用三光子激发荧光的ITF显微镜，在激发波长为1060 nm、成像深度为100 µm时，其空间分辨率为106 nm。
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