Park Hwayong, Hwang Youn-Hwan, Ma Jin Yeul
KM (Korean Medicine) Application Center, Korea Institute of Oriental Medicine, Daegu, Korea.
Integr Med Res. 2017 Jun;6(2):214-222. doi: 10.1016/j.imr.2017.03.006. Epub 2017 Jun 1.
The traditional medicine (OY) has been prescribed in East Asia for hundreds of years for the treatment of stroke, paralysis, and ataxia. OY also has therapeutic effects on arthralgia, myalgia, and rheumatoid arthritis, and recent studies have shown its protective effects against apoptosis of hippocampal cells and its anti-inflammatory effects on the peripheral blood cells of patient with cerebral infarction. Many studies have explored the use of traditional medicine and herb materials in the development of safe, novel, and effective pharmaceuticals with fewer side effects. These efforts commonly adopt a bioconversion tool for fermentation with beneficial microbes. However, only pharmaceuticals with high levels of safety and low levels of toxicity can be used in healthcare system.
OY water extract was fermented with and assayed for acute toxicity and genotoxicity. Single dose acute toxicity, bacterial reverse mutation, chromosome aberrations, and micronucleus were observed and assayed in rats, histidine/tryptophan auxotrophic bacteria, Chinese hamster ovary fibroblast cells, and mice bone marrow cells, respectively.
All the experimental animals showed no abnormal behavior, clinical signs, body weight increases, or mortality. In the bacterial cultures, no revertant colonies were observed. Morphological and numerical chromosomal aberrations were not found in all metaphases examined. Frequency of induced micronuclei was not significantly increased in all doses applied.
As a whole, no acute toxicity or genotoxicity were observed in all the assays examined. Therefore, fermented OY is considered to be a safe material that can be used for development of complementary and alternative medicine using bioconversion.
传统药物（OY）在东亚已被用于治疗中风、瘫痪和共济失调数百年。OY对关节痛、肌痛和类风湿性关节炎也有治疗作用，最近的研究表明其对海马细胞凋亡具有保护作用，对脑梗死患者外周血细胞具有抗炎作用。许多研究探索了利用传统药物和草药开发安全、新颖且副作用较少的有效药物。这些努力通常采用生物转化工具与有益微生物进行发酵。然而，只有安全性高、毒性低的药物才能用于医疗保健系统。
用OY水提取物进行发酵，并测定其急性毒性和遗传毒性。分别在大鼠、组氨酸/色氨酸营养缺陷型细菌、中国仓鼠卵巢成纤维细胞和小鼠骨髓细胞中观察和测定单剂量急性毒性、细菌回复突变、染色体畸变和微核。
所有实验动物均未表现出异常行为、临床体征、体重增加或死亡。在细菌培养物中，未观察到回复菌落。在所有检查的中期相中均未发现形态学和数量染色体畸变。在所有应用剂量下，诱导微核的频率均未显著增加。
总体而言，在所有检测中均未观察到急性毒性或遗传毒性。因此，发酵后的OY被认为是一种安全的物质，可用于通过生物转化开发补充和替代医学。
