Department of Life Science and Biotechnology, College of Natural Sciences, Dong-eui University, Busan, 614-714, Korea.
Arch Pharm Res. 2013 May;36(5):641-8. doi: 10.1007/s12272-013-0080-z. Epub 2013 Mar 6.
Aqueous extracts of the dried mature (ANP-W) and immature Citrus unshiu peels (CUP-W) have been used as a traditional folk medicine for the treatment of gastrointestinal (GI) motility disorders in Korea. In the present study, neither ANP-W nor CUP-W exhibited significant toxicity even at an oral dose of 5 g/kg to mice. The effects of ANP-W and CUP-W on GI motor function were investigated by measuring the intestinal transit rate (ITR) of Evans blue in normal mice and rats with experimental GI motility dysfunctions (GMDs). In normal mice, the ITR was significantly increased by ANP-W (0.1-1 g/kg) in a dose dependent manner, whereas CUP-W elicited no significant change. GMD was induced by appropriate surgery or an intraperitoneal injection of acetic acid to the rats. The ITR in the GMD rats was significantly retarded compared to that in normal rats. However, the retardation was significantly inhibited by ANP-W (0.1-1 g/kg) in a dose dependent manner. The above results suggest that ANP-W has the potential for development as a prokinetic agent that may prevent or alleviate GMD in human patients.
干制成熟（ANP-W）和未成熟柑橘果皮（CUP-W）的水提物已被用作韩国治疗胃肠道（GI）运动障碍的传统民间药物。在本研究中，即使以 5 g/kg 的口服剂量给予小鼠，ANP-W 和 CUP-W 均无明显毒性。通过测量正常小鼠和具有实验性胃肠道运动障碍（GMD）的大鼠中 Evans 蓝的肠传递率（ITR）来研究 ANP-W 和 CUP-W 对 GI 运动功能的影响。在正常小鼠中，ANP-W（0.1-1 g/kg）呈剂量依赖性显著增加 ITR，而 CUP-W 则没有明显变化。通过适当的手术或向大鼠腹腔内注射乙酸诱导 GMD。与正常大鼠相比，GMD 大鼠的 ITR 明显延迟。然而，ANP-W（0.1-1 g/kg）以剂量依赖性方式显著抑制了这种延迟。上述结果表明，ANP-W 具有开发成为促动力剂的潜力，可预防或缓解人类患者的 GMD。
