Pandya Urja, Prakash Sunita, Shende Kishor, Dhuldhaj Umesh, Saraf Meenu
Department of Microbiology and Biotechnology, School of Sciences, Gujarat University, Ahmedabad, 380 009, India.
Molecular Biophysics Unit, Indian Institute of Science, Bangalore, 560012, India.
3 Biotech. 2017 Jul;7(3):175. doi: 10.1007/s13205-017-0827-1. Epub 2017 Jun 29.
A potential antagonist, designated strain Bacillus subtilis MBCU5 was previously isolated from vermicompost-amended soils of Gandhinagar, Gujarat, India. Crude allelochemicals from strain MBCU5 displayed strong antifungal activity against Macrophomina phaseolina as well as Rhizoctonia solani. These crude allelochemicals were tentatively identified as iturin, fengycin and surfactin through TLC and HPTLC analysis. Lipopeptides produced by MBCU5 were identified by MALDI-TOF-MS and LC-ESI-MS/MS analysis showed that iturin homologues (m/z 1020-1120), surfactin (m/z 1008.7 and m/z 1022.7), fengycin A and fengycin B (m/z 1400-1550) types of allelochemicals which are responsible for antifungal activity against pathogens. PCR analysis showed presence of genes (i.e. Iturin A synthetase KJ531680 and Surfactin synthetase KJ601726) involved in the biosynthesis of allelochemicals. Many reports showed lipopeptides from Bacillus species; this is the first report executed of multifarious allelochemicals from vermicompost-amended soil due to the presence of predominant Bacillus species.
一种潜在的拮抗剂，命名为枯草芽孢杆菌MBCU5，先前是从印度古吉拉特邦甘地讷格尔经蚯蚓堆肥改良的土壤中分离出来的。来自菌株MBCU5的粗化感物质对菜豆壳球孢菌和立枯丝核菌均表现出强烈的抗真菌活性。通过薄层色谱（TLC）和高效薄层色谱（HPTLC）分析，这些粗化感物质初步被鉴定为伊枯草菌素、丰原素和表面活性素。通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）鉴定了MBCU5产生的脂肽，液相色谱-电喷雾串联质谱（LC-ESI-MS/MS）分析表明，伊枯草菌素同系物（质荷比1020-1120）、表面活性素（质荷比1008.7和质荷比1022.7）、丰原素A和丰原素B（质荷比1400-1550）等类型的化感物质具有抗病原体的抗真菌活性。聚合酶链反应（PCR）分析表明存在参与化感物质生物合成的基因（即伊枯草菌素A合成酶KJ531680和表面活性素合成酶KJ601726）。许多报道显示了芽孢杆菌属的脂肽；这是由于优势芽孢杆菌属的存在，首次报道了来自蚯蚓堆肥改良土壤的多种化感物质。
