Debbarma Prasenjit, Raghuwanshi Shikha, Singh Jyoti, Suyal Deep Chandra, Zaidi M G H, Goel Reeta
Department of Microbiology, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, 263145, India.
Department of Chemistry, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, 263145, India.
3 Biotech. 2017 Jul;7(3):178. doi: 10.1007/s13205-017-0789-3. Epub 2017 Jun 29.
Application of polyhydroxybutyrate (PHB) to plastic industry has expanded over the last decades due to its attracting features over petro-based plastic, and therefore, its waste accumulation in nature is inevitable. In the present study, a total of four bacterial strains, viz., MK3, PN12, PW1, and Lna3, were formulated into a consortium and subsequently used as biological tool for degradation of biopolymers. The consortium was tested through λ shifts under in vitro conditions for utilization of PHB as sole carbon source. Talc-based bioformulations of consortium were used for the degradation of PHB film composites under in situ conditions. After 9 months of incubation, the recovered samples were monitored through Fourier transform infrared (FT-IR) spectroscopy and scanning electron microscopy (SEM), respectively. Analytical data, viz., changes in λ shifts (212-219 nm), FT-IR spectra, and SEM micrographs, revealed the biodegradation potential of developed consortium against PHB film composites, i.e., higher degradation of copolymer films was found over blend films. The used consortium had enhanced the rate of natural degradation and can be further used as a natural tool to maintain and restore global environmental safety.
在过去几十年里，聚羟基丁酸酯（PHB）因其相对于石油基塑料具有吸引人的特性，在塑料工业中的应用不断扩大，因此，其在自然界中的废物积累不可避免。在本研究中，总共四种细菌菌株，即MK3、PN12、PW1和Lna3，被配制成一个混合菌群，随后用作生物聚合物降解的生物工具。该混合菌群在体外条件下通过λ位移测试，以利用PHB作为唯一碳源。基于滑石粉的混合菌群生物制剂用于原位条件下PHB薄膜复合材料的降解。培养9个月后，分别通过傅里叶变换红外（FT-IR）光谱和扫描电子显微镜（SEM）对回收的样品进行监测。分析数据，即λ位移（212-219nm）的变化、FT-IR光谱和SEM显微照片，揭示了所开发的混合菌群对PHB薄膜复合材料的生物降解潜力，即共聚物薄膜的降解程度高于共混薄膜。所使用的混合菌群提高了自然降解速率，可进一步用作维护和恢复全球环境安全的天然工具。
