弓形虫抗原的无细胞合成

Cell free synthesis of Toxoplasma gondii antigens.

作者信息

Prince J B, Koven-Quinn M A, Remington J S, Sharma S D

出版信息

Mol Biochem Parasitol. 1985 Nov;17(2):163-70. doi: 10.1016/0166-6851(85)90015-5.

DOI:10.1016/0166-6851(85)90015-5

Abstract

Functionally active poly(A)-containing mRNA was isolated from tachyzoites of the RH strain of Toxoplasma gondii. The T. gondii mRNA was capable of directing the synthesis of proteins in a wheat germ in vitro translation system, but not in a cell free system derived from rabbit reticulocyte lysate. Efficient translation in the wheat germ system required spermine and exogenous tRNA. Amino acid incorporation was maximal at 110 mM K+ and 1.8 mM Mg2+. Tachyzoite antigens synthesized in vitro were immunoprecipitated with T. gondii antibodies from rabbits, mice and humans. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of immunoprecipitated polypeptides yielded patterns that differed according to antibody source, but all T. gondii antibody preparations reacted with a translation product with an apparent molecular weight of 24 000.

摘要

从刚地弓形虫RH株速殖子中分离出具有功能活性的含多聚腺苷酸的mRNA。刚地弓形虫mRNA能够在小麦胚体外翻译系统中指导蛋白质合成，但不能在源自兔网织红细胞裂解物的无细胞系统中指导蛋白质合成。在小麦胚系统中高效翻译需要精胺和外源性tRNA。在110 mM K⁺和1.8 mM Mg²⁺时氨基酸掺入量最大。体外合成的速殖子抗原用来自兔、小鼠和人类的刚地弓形虫抗体进行免疫沉淀。免疫沉淀多肽的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳产生的图谱因抗体来源而异，但所有刚地弓形虫抗体制剂都与一种表观分子量为24000的翻译产物发生反应。

相似文献

1

Cell free synthesis of Toxoplasma gondii antigens.弓形虫抗原的无细胞合成

Mol Biochem Parasitol. 1985 Nov;17(2):163-70. doi: 10.1016/0166-6851(85)90015-5.

2

Characterization and in vitro translation of Toxoplasma gondii ribonucleic acid.刚地弓形虫核糖核酸的特性鉴定与体外翻译

Mol Biochem Parasitol. 1986 Mar;18(3):313-20. doi: 10.1016/0166-6851(86)90088-5.

3

Wheat embryo ribonucleates. XIV. Mass isolation of mRNA from wheat germ and comparison of its translational capacity with that of mRNA from imbibing wheat embryos.小麦胚核糖核酸。十四。从小麦胚芽中大规模分离信使核糖核酸及其翻译能力与吸胀小麦胚信使核糖核酸的比较。

Can J Biochem. 1979 Sep;57(9):1170-5. doi: 10.1139/o79-151.

4

Translation of poly(riboadenylic acid)-enriched messenger RNAs from the yeast, Saccharomyces cerevisiae, in heterologous cell-free systems.从酵母酿酒酵母中提取的富含聚（核糖腺苷酸）的信使核糖核酸在异源无细胞系统中的翻译

Biochemistry. 1975 Mar 11;14(5):1038-46. doi: 10.1021/bi00676a024.

5

The effect of aging on the structure and function of liver messenger RNA.衰老对肝脏信使核糖核酸结构和功能的影响。

Mech Ageing Dev. 1985 Oct 14;32(1):99-111. doi: 10.1016/0047-6374(85)90039-9.

6

Translation of partially purified poly(A)+ protamine messenger RNA components in wheat germ and rabbit reticulocyte cell-free systems. Evidence for translational control mechanisms.小麦胚芽和兔网织红细胞无细胞体系中部分纯化的聚腺苷酸（poly(A)+）鱼精蛋白信使核糖核酸（mRNA）组分的翻译。翻译控制机制的证据。

Biochim Biophys Acta. 1979 May 24;562(3):481-94. doi: 10.1016/0005-2787(79)90111-4.

7

A direct evidence for the involvement of poly(A) in protein synthesis.聚腺苷酸（poly(A)）参与蛋白质合成的直接证据。

Biochem Biophys Res Commun. 1987 Apr 29;144(2):649-56. doi: 10.1016/s0006-291x(87)80015-3.

8

Trypanosoma cruzi: characterization of in vitro and in vivo synthesized polypeptides from epimastigote forms of different strains.

Exp Parasitol. 1987 Dec;64(3):354-60. doi: 10.1016/0014-4894(87)90047-6.

9

A special repressor/activator system controls distribution of mRNA between translationally active and inactive mRNPs in rabbit reticulocytes.

FEBS Lett. 1989 Dec 4;258(2):227-9. doi: 10.1016/0014-5793(89)81659-x.

10

Immunoenzymatic detection of three kinds of 43,000-molecular-weight antigens by monoclonal antibodies in the insoluble fraction of Toxoplasma gondii.用单克隆抗体对弓形虫不溶性部分中三种43000分子量抗原进行免疫酶检测。

Infect Immun. 1984 Mar;43(3):1047-53. doi: 10.1128/iai.43.3.1047-1053.1984.

引用本文的文献

1

Expression, characterization, and serologic reactivity of recombinant surface antigen P22 of Toxoplasma gondii.刚地弓形虫重组表面抗原P22的表达、特性鉴定及血清学反应性

J Clin Microbiol. 1992 May;30(5):1127-33. doi: 10.1128/jcm.30.5.1127-1133.1992.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

文档翻译

学术文献翻译模型，支持多种主流文档格式。