Rubin H N, Halim M N
Biochem Biophys Res Commun. 1987 Apr 29;144(2):649-56. doi: 10.1016/s0006-291x(87)80015-3.
A radioactive polyadenylated globin mRNA was translated in either rabbit reticulocyte lysate or wheat germ extract under various conditions. When globin mRNA was translated, globin synthesis was directly proportional to the rate of loss in A units from the poly(A) tail. On the other hand, when globin poly(A) mRNA was incubated under non-translated conditions, no loss of A units was detected. The presence of ribonuclease inhibitor in the reaction mixture did not alter either the rate of globin synthesis or the loss in A units from the poly(A) tail. The present data suggests a correlation between protein synthesis and loss in A units from the poly(A) tail.
一种放射性多聚腺苷酸化的珠蛋白mRNA在不同条件下于兔网织红细胞裂解物或小麦胚芽提取物中进行翻译。当珠蛋白mRNA进行翻译时，珠蛋白的合成与多聚（A）尾中A单位的丢失速率直接成正比。另一方面，当珠蛋白多聚（A）mRNA在非翻译条件下孵育时，未检测到A单位的丢失。反应混合物中核糖核酸酶抑制剂的存在既没有改变珠蛋白的合成速率，也没有改变多聚（A）尾中A单位的丢失情况。目前的数据表明蛋白质合成与多聚（A）尾中A单位的丢失之间存在相关性。
