Valadan Reza, Nejatollahi Foroogh, Ehsani-Nori Hoda, Habibi Hassan, Amini Hamid, Aliabadian Mansour
Molecular and Cell Biology Research Center, Department of Immunology, Mazandaran University of Medical Sciences, Sari, Iran.
Recombinant Antibody Laboratory, Department of Immunology, Graduate School for Advanced Biomedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran.
Zoo Biol. 2017 Jul;36(4):289-293. doi: 10.1002/zoo.21363. Epub 2017 Jun 30.
Global environmental change and rapid destruction of natural habitats necessitate the conservation of endangered and threatened birds of prey. Recently, molecular sex identification methods based on amplification of introns of chromodomain-helicase DNA binding protein1 (CHD1) have provided valuable tools for ecological study and conservation breeding programs of birds. These methods employ a primer pair flanking an intron which varies considerably in length between the avian gametologs CHD1Z and CHD1W. Herein, we test the applicability of CHD1Z and CHD1W as universal tags for molecular sex identification in birds of prey of Iran. We showed successful sex identification in 22 species of birds of prey using feathers as the source of DNA. The results suggest that the regions of CHD1W and CHD1Z amplified in this study are conserved among most of Falconiformes, enabling accurate sex identification in birds of prey.
全球环境变化和自然栖息地的迅速破坏使得保护濒危和受威胁的猛禽成为必要。最近，基于对染色体结构域解旋酶DNA结合蛋白1（CHD1）内含子进行扩增的分子性别鉴定方法，为鸟类的生态学研究和保护育种计划提供了有价值的工具。这些方法使用一对引物，该引物位于一个内含子两侧，该内含子在鸟类性染色体同源基因CHD1Z和CHD1W之间的长度差异很大。在此，我们测试CHD1Z和CHD1W作为伊朗猛禽分子性别鉴定通用标签的适用性。我们利用羽毛作为DNA来源，成功鉴定了22种猛禽的性别。结果表明，本研究中扩增的CHD1W和CHD1Z区域在大多数隼形目鸟类中是保守的，能够准确鉴定猛禽的性别。
