Kretschmer R, Collado M L, Pacheco M G, Salinas M C, López-Osuna M, Lecuona M, Castro E M, Arellano J
Parasite Immunol. 1985 Sep;7(5):527-43. doi: 10.1111/j.1365-3024.1985.tb00097.x.
The supernatant fluid of axenically grown E. histolytica inhibits chemotaxis, chemokinesis and random mobility of human mononuclear phagocytes (MP) as measured in Boyden chambers. Human polymorphonuclear phagocytes (PMN) locomotion is apparently unaffected. The factor was found in comparable amounts in the supernatant fluid of axenic cultures of four E. histolytica strains that differed in their human pathogenicity and virulence, as well as in two entamoebas non-pathogenic for man. This dialysable and thermolabile MP-locomotion inhibiting entamoeba product (EP) can be absorbed out by incubation with MP, but not with lymphocytes, while partial absorption was observed using PMN. The MP-locomotion inhibitory effect of this EP was cancelled by inhibiting protein synthesis in the MP by means of cycloheximide. In vivo, this EP caused a delay in MP migration in Rebuck skin windows. The molecular weight of this EP lies between 478 and 765 by gel-sieve chromatography. Our results suggest a direct effect upon the cytoskeletal and locomotive apparatus of the MP. This MP-locomotion inhibiting EP could contribute to the paucity of the inflammatory reaction observed in the advanced stages of invasive amoebiasis and consequently also to the lack of scar tissue formation upon healing of amoebic lesions.
在博伊登小室实验中，无菌培养的溶组织内阿米巴的上清液会抑制人类单核吞噬细胞（MP）的趋化性、化学增活现象和随机移动。人类多形核吞噬细胞（PMN）的移动显然未受影响。在四种人类致病性和毒力不同的溶组织内阿米巴菌株以及两种对人类无致病性的内阿米巴的无菌培养上清液中，发现了含量相当的该因子。这种可透析且对热不稳定的抑制MP移动的阿米巴产物（EP），与MP共同孵育时可被吸收，但与淋巴细胞共同孵育时则不会，而与PMN共同孵育时会出现部分吸收现象。通过放线菌酮抑制MP中的蛋白质合成后，该EP对MP移动的抑制作用被消除。在体内，这种EP会导致Rebuck皮窗实验中MP迁移延迟。通过凝胶过滤色谱法测定，这种EP的分子量在478至765之间。我们的研究结果表明，它对MP的细胞骨架和移动装置有直接影响。这种抑制MP移动的EP可能是侵袭性阿米巴病晚期炎症反应较少的原因，因此也可能是阿米巴病变愈合后缺乏瘢痕组织形成的原因。
