Fang Chengnan, Wang Qingqing, Liu Xinyu, Xu Guowang
CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; University of Chinese Academy of Sciences, Beijing 100049, China.
CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; University of Chinese Academy of Sciences, Beijing 100049, China.
J Pharm Biomed Anal. 2017 Oct 25;145:158-168. doi: 10.1016/j.jpba.2017.06.046. Epub 2017 Jun 20.
Siraitia grosvenorii is an economic and medicinal plant, its fruit is considered to be good to health for its diverse bioactive ingredients. However, the clarification of chemical composition and their changes after saccharification procedure are not well performed. In present study, a metabolomics method based on ultra-high-performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry was developed for metabolic profiling acquisition of Siraitia grosvenorii extract. Furthermore, information dependent analysis (IDA) combined with self-constructed LC-MS/MS identification system for metabolites were employed to identify primary and secondary metabolites in Siraitia grosvenorii. A total of 126 metabolites were identified or tentatively identified. The obvious differences of metabolic profiling between green fruit and saccharified yellow fruit were observed, and metabolites showed their own distribution characteristics in peel, flesh and seed. The majority of the nutrients and effective components were more distributed in flesh and peel, and saccharification was conducive to accumulation of sweet glycosides. This study not only expanded metabolite composition information of Siraitia grosvenorii, but also specified distribution characteristics of identified metabolites.
罗汉果是一种经济药用植物，其果实因其多种生物活性成分而被认为对健康有益。然而，其化学成分及其糖化过程后的变化尚未得到充分阐明。在本研究中，建立了一种基于超高效液相色谱串联四极杆飞行时间质谱的代谢组学方法，用于罗汉果提取物的代谢谱采集。此外，采用信息依赖分析（IDA）结合自行构建的LC-MS/MS代谢物鉴定系统，对罗汉果中的初级和次级代谢物进行鉴定。共鉴定或初步鉴定出126种代谢物。观察到绿色果实和糖化黄色果实之间代谢谱的明显差异，代谢物在果皮、果肉和种子中呈现出各自的分布特征。大多数营养成分和有效成分在果肉和果皮中分布较多，糖化有利于甜味糖苷的积累。本研究不仅扩展了罗汉果的代谢物组成信息，还明确了已鉴定代谢物的分布特征。
