School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Mar 15;1079:25-33. doi: 10.1016/j.jchromb.2018.02.002. Epub 2018 Feb 6.
Mogroside V, a natural compound isolated from the fruits of Siraitia grosvenorii (Swingle), is a promising candidate for anti-diabetic activity. The present study aims to develop a simple and practical strategy for comparing the in vivo metabolite profiling of mogroside V in healthy and type 2 diabetic (T2D) model rats. In this paper, a highly sensitive and rapid ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) with MetaboLynx™ software combined with mass defect filtering (MDF) method was established and successfully applied to detect and identify the metabolites in plasma, urine, bile and feces samples of healthy and model rats administrated with mogroside V. The distribution of metabolites in plasma, bile, urine and feces of healthy and model rats had obvious differences. A total of 23 metabolites were observed in healthy rats while 26 metabolites were detected in model rats. The results indicated that dehydrogenation, deoxidation, oxidation and isomerization were the major metabolic transformations of mogroside V. Additionally, it was noticed that the peak areas of metabolites in T2D rat plasma samples were much larger than those of metabolites in healthy rat plasma sample, whereas in T2D rat urine samples they were remarkably smaller compared with healthy rat urine sample. These high blood concentrations of metabolites might be beneficial for the treatment of T2D. The results of this study are valuable and important in understanding the metabolic process and therapeutic mechanism of mogroside V.
罗汉果甜苷 V 是从罗汉果（Swingle）果实中分离得到的一种天然化合物，具有抗糖尿病活性的潜力。本研究旨在开发一种简单实用的策略，用于比较罗汉果甜苷 V 在健康和 2 型糖尿病（T2D）模型大鼠体内的代谢物谱。本文建立了一种高灵敏度和快速的超高效液相色谱/四极杆飞行时间质谱（UPLC-Q-TOF/MS）联用 MetaboLynx™ 软件结合质量亏损过滤（MDF）方法，成功地用于检测和鉴定健康和模型大鼠给予罗汉果甜苷 V 后血浆、尿液、胆汁和粪便样品中的代谢物。健康和模型大鼠血浆、胆汁、尿液和粪便中代谢物的分布有明显差异。在健康大鼠中观察到 23 种代谢物，而在模型大鼠中检测到 26 种代谢物。结果表明，罗汉果甜苷 V 的主要代谢转化途径为脱氢、脱氧、氧化和异构化。此外，还注意到 T2D 大鼠血浆样品中代谢物的峰面积明显大于健康大鼠血浆样品中代谢物的峰面积，而 T2D 大鼠尿液样品中代谢物的峰面积明显小于健康大鼠尿液样品中代谢物的峰面积。这些高血浓度的代谢物可能有益于 T2D 的治疗。本研究的结果对于理解罗汉果甜苷 V 的代谢过程和治疗机制具有重要的价值和意义。
