Li Wen-Rong, He San-Gang, Liu Chen-Xi, Zhang Xue-Mei, Wang Li-Qin, Lin Jia-Peng, Chen Lei, Han Bin, Huang Jun-Cheng, Liu Ming-Jun
College of Life Science and Technology, Xinjiang University, No. 14 Shengli road, Urmuqi, Xinjiang, China; Key Laboratory of Genetics, Breeding & Reproduction of Grass-Feeding Livestock, Ministry of Agriculture, No. 468 Alishan road, Urmuqi, Xinjiang, China; Key Laboratory of Animal Biotechnology of Xinjiang Institute of Animal Biotechnology, Xinjiang Academy of Animal Science, No. 468 Alishan road, Urmuqi, Xinjiang, China.
Key Laboratory of Genetics, Breeding & Reproduction of Grass-Feeding Livestock, Ministry of Agriculture, No. 468 Alishan road, Urmuqi, Xinjiang, China; Key Laboratory of Animal Biotechnology of Xinjiang Institute of Animal Biotechnology, Xinjiang Academy of Animal Science, No. 468 Alishan road, Urmuqi, Xinjiang, China.
Gene. 2017 Sep 5;627:477-483. doi: 10.1016/j.gene.2017.06.037. Epub 2017 Jun 28.
Fibroblast growth factor 5 (FGF5) has been recognized as an inhibitor to cease animal hair growth, while in contrary, FGF5 short alternative transcript (FGF5s) can induce hair growth by antagonizing FGF5 function. To investigate the role of FGF5s in wool growth in Chinese Merino sheep, we generated transgenic sheep of ectopic expression of FGF5s by injection of recombinant lentivirus into zygote. Totally 20 transgenic sheep were obtained and 12 were alive after birth. Characterization of the transgene revealed that the transgenic sheep showed variety of integrant, ranged from 2 to 11 copies of transgene. The ectopic expression of FGF5s was observed in all transgenic sheep. Further study on the effect of ectopic expression of FGF5s revealed that the wool length of transgenic sheep were significantly longer than that of non-transgenic control, with 9.17cm of transgenic lambs versus 7.58cm of control animals. Notably, besides the increase of wool length, the yearling greasy fleece weight was also concordantly greater than that of wild-type (p<0.01), with 3.22kg of transgenic sheep versus 2.17kg of control lambs (p<0.01) in average. Our results suggested that overexpression of FGF5s could stimulate wool growth and resulted in increase of wool length and greasy wool weight.
成纤维细胞生长因子5（FGF5）被认为是一种抑制动物毛发生长的因子，而相反，FGF5短可变转录本（FGF5s）可通过拮抗FGF5的功能来诱导毛发生长。为了研究FGF5s在中国美利奴羊羊毛生长中的作用，我们通过向受精卵注射重组慢病毒来制备FGF5s异位表达的转基因羊。共获得20只转基因羊，出生后存活12只。对转基因的鉴定表明，转基因羊呈现出多种整合情况，转基因拷贝数从2个到11个不等。在所有转基因羊中均观察到FGF5s的异位表达。对FGF5s异位表达效果的进一步研究表明，转基因羊的羊毛长度显著长于非转基因对照，转基因羔羊的羊毛长度为9.17厘米，而对照动物为7.58厘米。值得注意的是，除了羊毛长度增加外，一岁龄的污毛量也一致地高于野生型（p<0.01），转基因羊平均为3.22千克，而对照羔羊平均为2.17千克（p<0.01）。我们的结果表明，FGF5s的过表达可刺激羊毛生长，并导致羊毛长度和污毛量增加。
