Janaki Ramaiah M, Naushad Shaik Mohammad, Lavanya A, Srinivas Chatla, Anjana Devi Tangutur, Sampathkumar Sowganthika, Dharan Dhepikha Bharani, Bhadra Manika Pal
School of Chemical and Biotechnology, SASTRA University, Thanjavur 613401, India.
School of Chemical and Biotechnology, SASTRA University, Thanjavur 613401, India; Sandor Life Sciences Pvt. Ltd, Banjara Hills, Road No: 3, Hyderabad-500034, India.
Gene. 2017 Sep 5;627:379-386. doi: 10.1016/j.gene.2017.06.031. Epub 2017 Jun 29.
Scriptaid (SCR), a well-known histone deacetylase inhibitor, cause various cellular effects such as cell growth inhibition and apoptosis. In this study, we have evaluated the anti-cancer effects of Scriptaid in HeLa cells, IMR-32 and HepG2 cells. Scriptaid inhibited the growth of HeLa cells with IC of 2μM at 48h in a dose-dependent manner. Flow-cytometric analysis indicated that SCR induced apoptosis. Scriptaid was found to inhibit HDAC-8 effectively than other HDAC inhibitor such as TSA as observed by HDAC-8 assay, Western blotting and modelling study. This observation was further strengthened by an artificial neuronal network (ANN) model.
司立通（SCR）是一种著名的组蛋白去乙酰化酶抑制剂，可引起多种细胞效应，如细胞生长抑制和凋亡。在本研究中，我们评估了司立通对人宫颈癌HeLa细胞、人神经母细胞瘤IMR-32细胞和人肝癌HepG2细胞的抗癌作用。司立通以剂量依赖的方式抑制HeLa细胞的生长，48小时时的半数抑制浓度（IC）为2μM。流式细胞术分析表明，司立通诱导细胞凋亡。通过组蛋白去乙酰化酶8（HDAC-8）测定、蛋白质印迹法和模型研究发现，司立通比其他HDAC抑制剂（如曲古抑菌素A（TSA））更有效地抑制HDAC-8。人工神经网络（ANN）模型进一步证实了这一观察结果。
