肌腱蛋白-X对周围神经血管形成的潜在作用。

A potential contribution of tenascin-X to blood vessel formation in peripheral nerves.

作者信息

Sakai Hiromichi, Yokota Shigefumi, Kajitani Naoyo, Yoneyama Tsunao, Kawakami Kohei, Yasui Yukihiko, Matsumoto Ken-Ichi

机构信息

Department of Biosignaling and Radioisotope Experiment, Interdisciplinary Center for Science Research, Organization for Research and Academic Information, Shimane University, Japan.

Department of Anatomy and Morphological Neuroscience, Shimane University School of Medicine, Japan.

出版信息

Neurosci Res. 2017 Nov;124:1-7. doi: 10.1016/j.neures.2017.06.003. Epub 2017 Jun 28.

DOI:10.1016/j.neures.2017.06.003

PMID:28668501

Abstract

Tenascin-X (TNX), an extracellular matrix protein, is abundantly expressed in peripheral nerves. However, the physiological role of TNX in peripheral nerves remains unknown. In this study, we found that actin levels in sciatic nerves of TNX-deficient mice were markedly decreased. Since actin was highly expressed in endothelial cells in wild-type sciatic nerves, we assessed morphological alterations of blood vessels in TNX-null sciatic nerves. The density of blood vessels was significantly decreased and the size of blood vessels was larger than those in wild-type sciatic nerves. Immunofluorescence showed that TNX was expressed by Schwann cells and fibroblasts in sciatic nerves. The results suggest that TNX secreted from Schwann cells and/or fibroblasts is involved in blood vessel formation in peripheral nerves.

摘要

腱生蛋白-X（TNX）是一种细胞外基质蛋白，在外周神经中大量表达。然而，TNX在外周神经中的生理作用尚不清楚。在本研究中，我们发现TNX缺陷小鼠坐骨神经中的肌动蛋白水平显著降低。由于野生型坐骨神经的内皮细胞中肌动蛋白高度表达，我们评估了TNX基因敲除的坐骨神经中血管的形态学改变。血管密度显著降低，且血管大小比野生型坐骨神经中的更大。免疫荧光显示，TNX由坐骨神经中的雪旺细胞和成纤维细胞表达。结果表明，雪旺细胞和/或成纤维细胞分泌的TNX参与外周神经的血管形成。