Department of Occupational and Environmental Health Science, School of Public Health, Baotou Medical College, Baotou, Inner Mongolia Autonomous Region 014030, China; Department of Occupational and Environmental Health Science, School of Public Health, Peking University, Beijing 100191, China.
Department of Occupational and Environmental Health Science, School of Public Health, Baotou Medical College, Baotou, Inner Mongolia Autonomous Region 014030, China.
Environ Toxicol Pharmacol. 2017 Sep;54:21-27. doi: 10.1016/j.etap.2017.06.012. Epub 2017 Jun 20.
Black carbon (BC) and polycyclic aromatic hydrocarbons (PAHs), which are major atmospheric pollutants in China, are hazardous to humans following inhalation. BC can be oxidized by PAHs forming secondary particles of which the health effects are unknown. In this study, carbon black (CB) was used to simulate BC to reveal the adverse effects of 1,4-naphthoquinone aged carbon black (CB/1,4-NQ) particles on the membrane of human bronchial epithelial (16HBE) cells. It was showed that, the cell viability, cell membrane fluidity, membrane potential and mitochondrial membrane potential were significantly decreased after 16HBE cells were treated with CB, 1,4-NQ or CB/1,4-NQ. Meanwhile, the cell membrane permeability and intracellular Ca concentration were increased. CB/1,4-NQ could induce more adverse effects on cell membrane than single CB treated, while less than 1,4-NQ. The results indicated that CB/1,4-NQ particles in the atmosphere may cause more damage to health, and the effects on cell membrane can be used to evaluate the early health effects of the particulate matter exposure.
黑碳（BC）和多环芳烃（PAHs）是中国大气中的主要污染物，吸入后对人体有害。BC 可以被 PAHs 氧化形成二次颗粒，其健康影响尚不清楚。在这项研究中，使用碳黑（CB）模拟 BC，以揭示 1,4-萘醌老化碳黑（CB/1,4-NQ）颗粒对人支气管上皮（16HBE）细胞膜的不良影响。结果表明，16HBE 细胞用 CB、1,4-NQ 或 CB/1,4-NQ 处理后，细胞活力、细胞膜流动性、膜电位和线粒体膜电位显著降低，同时，细胞膜通透性和细胞内 Ca 浓度增加。CB/1,4-NQ 对细胞膜的不良影响比单独用 CB 处理更大，但小于 1,4-NQ。结果表明，大气中的 CB/1,4-NQ 颗粒可能对健康造成更大的损害，而对细胞膜的影响可用于评估颗粒物暴露的早期健康影响。
