Zhang Quanli, Zheng Xiufen, Sun Qi, Shi Run, Wang Jie, Zhu Biqing, Xu Lin, Zhang Guangqin, Ren Binhui
Department of Clinical Pharmacy, China Pharmaceutical University, Nanjing, 210009, PR China; Jiangsu Key Laboratory of Molecular and Translational Cancer Research, Nanjing, 210009, PR China; Department of Thoracic Surgery, Jiangsu Cancer Hospital, Institute Affiliated to Nanjing Medical University, Cancer Institute of Jiangsu Province, Baiziting 42, Xuanwu District, Nanjing, 210009, PR China.
Jiangsu Key Laboratory of Molecular and Translational Cancer Research, Nanjing, 210009, PR China.
Biochem Biophys Res Commun. 2017 Sep 2;490(4):1189-1196. doi: 10.1016/j.bbrc.2017.06.180. Epub 2017 Jun 29.
By analyzing The Cancer Genome Atlas (TCGA) datasets, we discovered that the zinc finger protein 692 (ZNF692) were over-expressed in Lung adenocarcinoma (LUAD) tissues compared to adjacent non-tumor tissues (P < 0.0001). In this study, we investigated the function of ZNF692 in the progression of LUAD. We found that ZNF692 knockdown inhibited LUAD cells proliferation, migration, and invasion both in vitro and in vivo. And LUAD cell apoptosis was induced following the down-regulation of ZNF692. Our results show that ZNF692 is over-expressed in LUAD tissues compared to adjacent normal tissues, and hyper-expression of ZNF692 in LUAD is an independent risk factor for worse overall survival in LUAD patients (HR: 8.800, 95%CI: 1.082-71.560, P = 0.042) by Tissue Microarray stain assay (TMA). GO analysis indicated that most genes were enriched in metabolic process which were associated highly with ZNF692 levels. Collectively, our results suggested that ZNF692 may serve as a potential oncogene and biomarker in LUAD by influencing cell metabolism.
通过分析癌症基因组图谱（TCGA）数据集，我们发现与相邻的非肿瘤组织相比，锌指蛋白692（ZNF692）在肺腺癌（LUAD）组织中过表达（P < 0.0001）。在本研究中，我们调查了ZNF692在LUAD进展中的功能。我们发现，ZNF692基因敲低在体外和体内均抑制LUAD细胞的增殖、迁移和侵袭。并且ZNF692下调后诱导了LUAD细胞凋亡。我们的结果表明，与相邻正常组织相比，ZNF692在LUAD组织中过表达，并且通过组织芯片染色分析（TMA），LUAD中ZNF692的高表达是LUAD患者总体生存较差的独立危险因素（HR：8.800，95%CI：1.082 - 71.560，P = 0.042）。基因本体（GO）分析表明，大多数基因富集在与ZNF692水平高度相关的代谢过程中。总体而言，我们的结果表明，ZNF692可能通过影响细胞代谢而成为LUAD中的潜在癌基因和生物标志物。
