Liao Hui-Qi, Tian Liu, Yang Hui, Ma Ni, Zhang Chang-Jun, Diao Hong-Lu
1Reproductive Medicine Center, People's Hospital Affiliated to Hubei University of Medicine, 2School of Biomedical Engineering, Hubei University of Medicine, Shiyan 442000, China. E-mail:
Nan Fang Yi Ke Da Xue Xue Bao. 2017 Jun 20;37(6):730-736. doi: 10.3969/j.issn.1673-4254.2017.06.03.
To investigate the effect of BIX01294 (BIX), a methyltransferase inhibitor, on the migration and decidualization of the stromal cells in mouse uterus.
Mouse endometrial stromal cells were isolated and cultured from the uterus of pregnant mice on day 3.5 of gestation. The migration and decidualization of mouse endometrial stromal cells treated with BIX at different concentrations were observed with wound healing assay and real-time PCR.
The migration distance of mouse endometrial stromal cells increased as the BIX concentration increased within the range below 15 µmol/L. Compared with the control cells, the cells treated with BIX (15 µmol/L) showed significantly increased migration distances, but increasing BIX concentration to 20 µmol/L did not further increase the cell migration distance and began to cause cell death. Compared with the control cells, the BIX-treated stromal cells exhibited significantly down-regulated expression of Ehmt2 mRNA, and 15 µmol/L BIX caused inhibition of decidualization in the stromal cells.
Within a defined concentration range, BIX promotes the migration and inhibits decidualization of mouse uterine stromal cells by inhibiting the expression of Ehmt2 mRNA.
研究甲基转移酶抑制剂BIX01294（BIX）对小鼠子宫基质细胞迁移和蜕膜化的影响。
从妊娠第3.5天的孕鼠子宫中分离并培养小鼠子宫内膜基质细胞。采用划痕愈合试验和实时定量PCR观察不同浓度BIX处理的小鼠子宫内膜基质细胞的迁移和蜕膜化情况。
在低于15 μmol/L的范围内，小鼠子宫内膜基质细胞的迁移距离随BIX浓度的增加而增加。与对照细胞相比，用BIX（15 μmol/L）处理的细胞迁移距离显著增加，但将BIX浓度提高到20 μmol/L并未进一步增加细胞迁移距离，且开始导致细胞死亡。与对照细胞相比，BIX处理的基质细胞中Ehmt2 mRNA表达显著下调，15 μmol/L BIX可抑制基质细胞的蜕膜化。
在一定浓度范围内，BIX通过抑制Ehmt2 mRNA的表达促进小鼠子宫基质细胞的迁移并抑制其蜕膜化。
