Di Scala-Guenot D, Strosser M T, Mialhe P
Horm Metab Res. 1985 Nov;17(11):566-71. doi: 10.1055/s-2007-1013608.
Substances with Somatostatin-Like Immunoreactivity (SLI) were extracted using 2 N acetic acid, from the three pancreatic lobes and the intestine of the duck. The concentration of SLI was found to be very high in the pancreas (4.2 micrograms/g wet weight), the splenic lobe containing 80% of pancreatic SLI compared with 10% for the dorsal and 10% for the ventral lobes. SLI was equally distributed between duodenum, jejunum and ileum and between their mucosal and muscular layers. Chromatography of pancreatic extracts, using a Sephadex G-25 column, showed mainly the tetradecapeptide form (somatostatin-14, S-14) with a small amount of big somatostatin. Chromatography of intestinal extracts revealed three peaks with SLI: big somatostatin, somatostatin-28 (S-28) and S-14. The substance represented by the predominant peak was co-eluted with that of synthetic S-28. In normal ducks, portal plasma SLI corresponded to big somatostatin S-28 and S-14. After total pancreatectomy the S-14 form disappeared from portal plasma, whereas, when the intestinal blood vessels were ligatured, the S-28 form disappeared. We therefore hypothesize that in portal blood, S-14 has a mainly pancreatic origin, and S-28 a mainly intestinal origin.
用2N乙酸从鸭的三个胰腺叶和肠道中提取具有生长抑素样免疫反应性（SLI）的物质。发现胰腺中SLI的浓度非常高（4.2微克/克湿重），脾叶含有胰腺SLI的80%，而背叶和腹叶分别为10%。SLI在十二指肠、空肠和回肠之间以及它们的粘膜层和肌肉层之间均匀分布。使用葡聚糖凝胶G-25柱对胰腺提取物进行色谱分析，主要显示十四肽形式（生长抑素-14，S-14），伴有少量的大生长抑素。肠道提取物的色谱分析显示出三个具有SLI的峰：大生长抑素、生长抑素-28（S-28）和S-14。主要峰所代表的物质与合成S-28的物质共洗脱。在正常鸭中，门静脉血浆SLI对应于大生长抑素S-28和S-14。全胰腺切除术后，门静脉血浆中S-14形式消失，而当结扎肠血管时，S-28形式消失。因此，我们假设在门静脉血中，S-14主要来源于胰腺，而S-28主要来源于肠道。
