Baldissera F G, Nielsen O V, Holst J J
Regul Pept. 1985 Jul;11(3):251-62. doi: 10.1016/0167-0115(85)90057-6.
We studied the molecular forms of somatostatin-like immunoreactivity (SLI), newly released from isolated perfused preparations of the porcine antrum, stomach, pancreas and upper small intestine: Perfusion effluents were concentrated by Sep-Pak C-18 adsorption, eluted with ethanol, dessicated, and subjected to gel filtration with subsequent radioimmunoassays for somatostatin-14 and N-terminal somatostatin-28 immunoreactivity. All the SLI newly released from the stomach and antrum eluted at the position of somatostatin-14, and such was also the case for more than 95% of the SLI newly released from the pancreas, while 68 -/+ 7% and 75 -/+ 8% of the SLI newly released from the isolated perfused jejunum and ileum, respectively, corresponded to somatostatin-28. By reverse phase HPLC the identity of these peptides with synhetic somatostatin-14 and -28 was established.
我们研究了从猪胃窦、胃、胰腺和上段小肠的离体灌注标本中 newly released 的生长抑素样免疫反应性（SLI）的分子形式：灌注流出液通过Sep-Pak C-18吸附进行浓缩，用乙醇洗脱，干燥，并进行凝胶过滤，随后对生长抑素-14和N端生长抑素-28免疫反应性进行放射免疫测定。从胃和胃窦 newly released 的所有SLI在生长抑素-14的位置洗脱，从胰腺 newly released 的SLI中超过95%也是如此，而从离体灌注的空肠和回肠 newly released 的SLI分别有68±7%和75±8%与生长抑素-28相对应。通过反相高效液相色谱法确定了这些肽与合成的生长抑素-14和-28的一致性。 （注：原文中“newly released”表述不太准确，可能是“新释放的”之类意思，这里按字面翻译存疑词）
