甲氧基胺增强5-氟尿嘧啶对结肠癌细胞系HT29的放射增敏作用。
Methoxyamine Enhances 5-Fluorouracil-Induced Radiosensitization in Colon Cancer Cell Line HT29.
作者信息
Khoei Samideh, Poorabdollahi Roghayeh, Mostaar Ahmad, Faeghi Fariborz
机构信息
Razi Drug Research Centre, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Department of Medical Physics, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
出版信息
Cell J. 2017 Jul-Sep;19(2):283-291. doi: 10.22074/cellj.2016.4295. Epub 2017 Feb 22.
OBJECTIVE
This study intended to observe the effects of methoxyamine (Mx) on cytotoxic effects and DNA damage caused by 5-Fluorouracil (5-FU) in combination with gamma radiation in a human colon cancer cell line, HT29.
MATERIALS AND METHODS
In this experimental study, HT29 cells were cultured as a monolayer and treated with different concentrations of 5-FU along with 1 mM Mx for 24 hours. Next, the cells were irradiated with 2 Gy gamma radiation. After the treatments, we assessed for DNA damage, cytotoxicity, and viability by alkaline comet, clonogenic survival, and trypan blue dye exclusion assays.
RESULTS
Cytotoxicity and DNA damage increased with increasing 5-FU concentration. The 1 mM Mx concentration had no significant effect on cytotoxicity and DNA damage from 5-FU; however, it increased the cytotoxic and genotoxic effects of different concentrations of 5-FU when used in combination with 2 Gy gamma radiation.
CONCLUSION
Mx combined with 5-FU enhanced the radiosensitivity of colon cancer cells.
目的
本研究旨在观察甲氧基胺（Mx）对5-氟尿嘧啶（5-FU）联合γ射线照射在人结肠癌细胞系HT29中所引起的细胞毒性作用和DNA损伤的影响。
材料与方法
在本实验研究中，HT29细胞以单层培养，并与1 mM甲氧基胺一起用不同浓度的5-氟尿嘧啶处理24小时。接下来，细胞接受2 Gy的γ射线照射。处理后，我们通过碱性彗星试验、克隆形成存活试验和台盼蓝染料排除试验评估DNA损伤、细胞毒性和活力。
结果
细胞毒性和DNA损伤随5-氟尿嘧啶浓度的增加而增加。1 mM甲氧基胺浓度对5-氟尿嘧啶的细胞毒性和DNA损伤没有显著影响；然而，当与2 Gyγ射线联合使用时，它增加了不同浓度5-氟尿嘧啶的细胞毒性和遗传毒性作用。
结论
甲氧基胺与5-氟尿嘧啶联合使用增强了结肠癌细胞的放射敏感性。
Zotero 插件