Adesida Solayide A, Ezenta Cynthia C, Adagbada Ajoke O, Aladesokan Amudat A, Coker Akitoye O
Department of Microbiology, Faculty of Science, University of Lagos, Lagos, Nigeria.
Molecular Biology and Biotechnology Division, Nigerian Institute of Medical Research, Yaba, Lagos, Nigeria.
Afr J Infect Dis. 2017 Jun 8;11(2):83-89. doi: 10.21010/ajid.v11i2.11. eCollection 2017.
Enterococci are indigenous flora of the gastro-intestinal tracts of animals and humans. Recently, interest in two major species, and , has heightened because of their ability to cause serious infections and their intrinsic resistance to antimicrobials. This study was aimed at determining the prevalence of . and . in human faecal samples and evaluating the susceptibility of the isolates to antibiotics.
One hundred faecal samples were collected from apparently healthy individuals and analysed using conventionalbacteriological methods. The susceptibility profile of the isolates to nine antibiotics were determined using disk diffusion method.
Seventy-three (73) were phenotypically identified and 65 of the isolates were differentiated into 36 (55.4%) and 29 (44.6%) . Eight (8) isolates could not be identified by the conventional biochemical methods employed. No dual colonization by the and was observed and isolation rate was not dependent on sex of the participants. All the isolates were resistant to ceftriaxone, cefuroxime and ceftizoxime. exhibited resistance toerythromycin (88.9%), gentamicin (77.8%), amoxicillin-clavulanate (63.9%), ofloxacin (44.4%), teicoplanin (19.4%) and vancomycin (16.7%). showed the least resistance to vancomycin (13.8%) and teicoplanin (27.7%). Remarkable multiple antibiotic resistances to the classes of antibiotic tested were observed among the two species.
The high carriage rate of antibiotic resistant and in this study provides information on the local antibiotic patterns of our enterococci isolates thereby suggesting that they could present as important reservoir and vehicle for dissemination of resistant genes in our community.
肠球菌是动物和人类胃肠道的固有菌群。近来，由于两种主要菌种（粪肠球菌和屎肠球菌）能够引起严重感染且对抗菌药物具有内在抗性，人们对它们的关注有所增加。本研究旨在确定人类粪便样本中粪肠球菌和屎肠球菌的流行情况，并评估分离株对抗生素的敏感性。
从表面健康的个体收集100份粪便样本，采用常规细菌学方法进行分析。使用纸片扩散法测定分离株对9种抗生素的敏感性。
通过表型鉴定出73株肠球菌，其中65株分离株被区分为36株（55.4%）粪肠球菌和29株（44.6%）屎肠球菌。采用的常规生化方法无法鉴定8株分离株。未观察到粪肠球菌和屎肠球菌的双重定植情况，分离率与参与者的性别无关。所有分离株均对头孢曲松、头孢呋辛和头孢唑肟耐药。粪肠球菌对红霉素（88.9%）、庆大霉素（77.8%）、阿莫西林-克拉维酸（63.9%）、氧氟沙星（44.4%）、替考拉宁（19.4%）和万古霉素（16.7%）耐药。屎肠球菌对万古霉素（13.8%）和替考拉宁（27.7%）的耐药性最低。在这两种菌种中均观察到对所测试抗生素类别的显著多重耐药性。
本研究中抗生素耐药的粪肠球菌和屎肠球菌的高携带率提供了关于我们分离的肠球菌的当地抗生素模式的信息，从而表明它们可能是我们社区中耐药基因传播的重要储存库和载体。
