Hasannejad Bibalan Meysam, Eshaghi Morteza, Sadeghi Javad, Asadian Mahla, Narimani Tahmineh, Talebi Malihe
Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, IR Iran.
Department of Microbiology, School of Medicine, Tehran University of Medical Sciences , Tehran, IR Iran.
Int J Mol Cell Med. 2015 Fall;4(4):240-4.
Enterococci are Gram positive and catalase- negative cocci that are found in the gastrointestinal tract of mammals and birds, and are readily isolated from soil, surface and waters. The aim of this study was to discriminate between Enterococcus isolates based on repetitive element sequence based -PCR (Rep-PCR) with the BOXA2R primer and their antibiotics profile. Enterococci isolates were obtained from 180 fecal samples. The isolates were identified by biochemical reaction and specific identification was confirmed by PCR with species specific primers. All isolates were subjected to Rep typing and antimicrobial susceptibility tests. Rep-PCR analysis of 180 isolates revealed 93 REP types with forty-five single types (ST1 to ST45) and forty-eight common types (CT1 to 48). Antibiotic susceptibility tests exhibited that 53 (29.4%), 43 (23.8%), 11 (6.1%) and 9 (5%) were resistant to erythromycin, tetracycline, gentamicin and ciprofloxacin respectively but among the isolates, sixteen were multi drug resistant (MDR). These MDR isolates showed 11 Rep types with seven single types and four common types. In addition, 81.2% of MDR isolates were from male subjects and the average age of these persons was more than fifty years. This study showed that 56.2% of MDR isolates were homogeneous with 95 % similarity, and high rate of resistance to tetracycline and erythromycin (81.2%) were observed in these isolates. The concern about these normal flora isolates are the pathogenic potential of these bacteria through the horizontal transfer of antibiotic resistance and virulence genes.
肠球菌是革兰氏阳性、过氧化氢酶阴性的球菌，存在于哺乳动物和鸟类的胃肠道中，并且很容易从土壤、地表和水中分离出来。本研究的目的是基于使用BOXA2R引物的重复元件序列PCR（Rep-PCR）及其抗生素谱来区分肠球菌分离株。从180份粪便样本中获得肠球菌分离株。通过生化反应鉴定分离株，并使用种特异性引物通过PCR确认特异性鉴定。所有分离株都进行了Rep分型和抗菌药敏试验。对180株分离株的Rep-PCR分析显示有93种REP类型，其中45种单一类型（ST1至ST45）和48种常见类型（CT1至48）。抗生素药敏试验表明，分别有53株（29.4%）、43株（23.8%）、11株（6.1%）和9株（5%）对红霉素、四环素、庆大霉素和环丙沙星耐药，但在这些分离株中，有16株是多重耐药（MDR）。这些MDR分离株显示出11种Rep类型，其中7种单一类型和4种常见类型。此外，81.2%的MDR分离株来自男性受试者，这些人的平均年龄超过50岁。本研究表明，56.2%的MDR分离株具有95%的相似性，并且在这些分离株中观察到对四环素和红霉素的高耐药率（81.2%）。对这些正常菌群分离株的担忧在于这些细菌通过抗生素耐药性和毒力基因的水平转移所具有的致病潜力。
