Pan J, He S, Yin X, Li Y, Zhou C, Zou S
Department of Orthodontics and The State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Orthod Craniofac Res. 2017 Aug;20(3):146-151. doi: 10.1111/ocr.12190. Epub 2017 Jul 3.
To investigate the effect of lithium on alveolar bone formation during orthodontic retention in rats.
After 2 weeks of orthodontic tooth movement (OTM), 42 8-week-old male Wistar rats were randomly divided into two orthodontic retention groups: one without (control) and the other with LiCl treatment (LiCl group). Samples were collected on days 0, 3, 7 and 14 during the retention period. We evaluated the bone volume/total volume (BV/TV) ratio and new bone formation in the region of interests (ie, the root, the periodontal ligament and the adjacent alveolar bone around the distal buccal surface of the distal root of the maxillary first molar). We performed quantitative analyses, including histology, histomorphometry and immunohistochemistry to identify Runx2 and Osterix expression.
The density of trabecular bone, the quantity of osteoblasts and the expression of osteogenic markers, Runx2 and Osterix, were significantly higher in the LiCl group than in the control group during the orthodontic retention period.
LiCl enhances alveolar bone formation during orthodontic retention in rats.
研究锂对大鼠正畸保持期间牙槽骨形成的影响。
在正畸牙齿移动（OTM）2周后，将42只8周龄雄性Wistar大鼠随机分为两个正畸保持组：一组不进行处理（对照组），另一组进行LiCl处理（LiCl组）。在保持期的第0、3、7和14天采集样本。我们评估了骨体积/总体积（BV/TV）比值以及上颌第一磨牙远中根远中颊侧表面周围感兴趣区域（即牙根、牙周膜和相邻牙槽骨）的新骨形成情况。我们进行了定量分析，包括组织学、组织形态计量学和免疫组织化学，以鉴定Runx2和Osterix的表达。
在正畸保持期，LiCl组的小梁骨密度、成骨细胞数量以及成骨标志物Runx2和Osterix的表达均显著高于对照组。
LiCl可增强大鼠正畸保持期间的牙槽骨形成。
