Ruivo Andréa Karina, Calsa Bruno, Cancellara Matheus Gomez, Lima João Paulo Nascimento, da Silva Karla Rovaris, Esquisatto Marcelo Augusto Marretto, Santamaria-Jr Milton
Graduate Program in Odontology, University Center of the Hermínio Ometto Foundation - FHO, Araras, SP, Brazil.
Department of Internal Medicine, Faculty of Medical Sciences at State University of Campinas - UNICAMP, Campinas, SP, Brazil.
Eur J Oral Sci. 2024 Oct;132(5):e13014. doi: 10.1111/eos.13014. Epub 2024 Aug 19.
This study aimed to evaluate the effects of the estrogen depression during orthodontic tooth movement on alveolar bone microarchitecture and periodontal ligament. Female Wistar rats were divided into two groups, one consisting of non-ovariectomized animals subjected to orthodontic tooth movement, and one comprising ovariectomized animals subjected to orthodontic tooth movement. Micro-CT assessment of bone volume to total volume (BV/TV), total porosity, trabecular thickness (Tb.Th), trabecular number (Tb.N), and trabecular separation (Tb.Sp) in the alveolar bone of the orthodontically moved tooth was performed. Histomorphometric analyses were made in the periodontal ligament, and immunoexpression of RANK, RANKL, OPG, and TUNEL were quantified. Orthodontic tooth movement in the group of ovariectomized rats was faster than in non-ovariectomized animals. The alveolar bone area showed lower values of BV/TV and trabecular thickness, and higher bone porosity and trabeculae numbers in the ovariectomized rats. Histological analyses in the ovariectomized group revealed an increase in collagen fibers in the periodontal ligament. The apoptotic cell counts in the periodontal ligament were higher in the group of ovariectomized rats than in the sham-operated rats. Ovariectomy resulted in an increase in tooth movement and alteration of the alveolar bone microstructure in the first 7 day of orthodontic tooth movement, and in the presence of apoptotic cells in the periodontal ligament.
本研究旨在评估正畸牙齿移动过程中雌激素缺乏对牙槽骨微结构和牙周韧带的影响。将雌性Wistar大鼠分为两组，一组为接受正畸牙齿移动的未去卵巢动物，另一组为接受正畸牙齿移动的去卵巢动物。对正畸移动牙齿的牙槽骨进行骨体积与总体积之比（BV/TV）、总孔隙率、骨小梁厚度（Tb.Th）、骨小梁数量（Tb.N）和骨小梁间距（Tb.Sp）的显微CT评估。对牙周韧带进行组织形态计量学分析，并对RANK、RANKL、OPG和TUNEL的免疫表达进行定量。去卵巢大鼠组的正畸牙齿移动比未去卵巢动物更快。去卵巢大鼠的牙槽骨区域BV/TV和骨小梁厚度值较低，骨孔隙率和骨小梁数量较高。去卵巢组的组织学分析显示牙周韧带中胶原纤维增加。去卵巢大鼠组牙周韧带中的凋亡细胞计数高于假手术大鼠组。去卵巢导致正畸牙齿移动的第1周内牙齿移动增加和牙槽骨微结构改变，以及牙周韧带中出现凋亡细胞。
