Takazoe I, Itoyama T, Kadomura Y
J Dent Res. 1979 Jul;58(7):1709-13. doi: 10.1177/00220345790580071101.
Protoplast formation with Bacterionema matruchotii was studied turbidimetrically and microscopically. The protoplasts were inducible during culture by addition of glycine, lysozyme and Ficoll to a medium containing Tween-80 and polyethylene glycol.
采用比浊法和显微镜观察法研究了具核梭杆菌原生质体的形成。在含有吐温80和聚乙二醇的培养基中添加甘氨酸、溶菌酶和菲可,可在培养过程中诱导原生质体形成。