Trenin A S, Dudnik Iu V
Antibiotiki. 1984 Aug;29(8):572-5.
The conditions for preparation and regeneration of the protoplasts of M. olivoasterospora were developed. It was found that effective formation of the protoplasts required preliminary cultivation of M. olivoasterospora in the medium containing glycine in a concentration inhibiting its growth at least by 60-80 per cent. The strains studied markedly differed in their sensitivity to glycine and were highly sensitive to it. The efficacy of the protoplast formation depended on the culture age and increased with the use of the lytic enzyme 3 of Cytophaga dissolvens. The possibility and advisability of the use of prolonged lysis of the Micromonospora cell walls were shown. A rich organic medium was used for regeneration of the protoplasts.
开发了橄榄色小单孢菌原生质体的制备和再生条件。发现原生质体的有效形成需要将橄榄色小单孢菌预先在含有甘氨酸的培养基中培养，该培养基中甘氨酸的浓度至少抑制其生长60 - 80%。所研究的菌株对甘氨酸的敏感性明显不同，且对其高度敏感。原生质体形成的效率取决于培养物的菌龄，并且使用溶纤维丁酸弧菌的裂解酶3时效率会提高。结果表明了对小单孢菌细胞壁进行长时间裂解的可能性和可取性。使用丰富的有机培养基来进行原生质体的再生。
