Mochalov Konstantin E, Chistyakov Anton A, Solovyeva Daria O, Mezin Alexey V, Oleinikov Vladimir A, Vaskan Ivan S, Molinari Michael, Agapov Igor I, Nabiev Igor, Efimov Anton E
Laboratory of Nano-bioengineering, National Research Nuclear University "MEPhI" (Moscow Engineering Physics Institute), Kashirskoe highway 31, 115409 Moscow, Russian Federation; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Ulitsa Miklukho-Maklaya 16/10, 117997 Moscow, Russian Federation.
Laboratory of Nano-bioengineering, National Research Nuclear University "MEPhI" (Moscow Engineering Physics Institute), Kashirskoe highway 31, 115409 Moscow, Russian Federation; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Ulitsa Miklukho-Maklaya 16/10, 117997 Moscow, Russian Federation.
Ultramicroscopy. 2017 Nov;182:118-123. doi: 10.1016/j.ultramic.2017.06.022. Epub 2017 Jun 21.
In the past decade correlative microscopy, which combines the potentials of different types of high-resolution microscopies with a variety of optical microspectroscopy techniques, has been attracting increasing attention in material science and biological research. One of outstanding solutions in this area is the combination of scanning probe microscopy (SPM), which provides data on not only the topography, but also the spatial distribution of a wide range of physical properties (elasticity, conductivity, etc.), with ultramicrotomy, allowing 3D multiparametric examination of materials. The combination of SPM and ultramicrotomy (scanning probe nanotomography) is very appropriate for characterization of soft multicompound nanostructurized materials, such as polymer matrices and microstructures doped with different types of nanoparticles (magnetic nanoparticles, quantum dots, nanotubes, etc.), and biological materials. A serious problem of this technique is a lack of chemical and optical characterization tools, which may be solved by using optical microspectroscopy. Here, we report the development of an instrumental approach to combining confocal microspectroscopy and 3D scanning probe nanotomography in a single apparatus. This approach retains all the advantages of SPM and upright optical microspectroscopy and allows 3D multiparametric characterization using both techniques. As the first test of the system developed, we have performed correlative characterization of the morphology and the magnetic and fluorescent properties of fluorescent magnetic microspheres doped with a fluorescent dye and magnetic nanoparticles. The results of this study can be used to obtain 3D volume images of a specimen for most high-resolution near-field scanning probe microscopies: SNOM, TERS, AFM-IR, etc. This approach will result in development of unique techniques combining the advantages of SPM (nanoscale morphology and a wide range of physical parameters) and high-resolution optical microspectroscopy (nanoscale chemical mapping and optical properties) and allowing simultaneous 3D measurements.
在过去十年中，相关显微镜技术将不同类型的高分辨率显微镜的潜力与多种光学显微光谱技术相结合，在材料科学和生物学研究中受到越来越多的关注。该领域的一个杰出解决方案是扫描探针显微镜（SPM）与超薄切片术的结合，前者不仅能提供形貌数据，还能提供多种物理性质（弹性、导电性等）的空间分布数据，后者则能对材料进行三维多参数检测。SPM与超薄切片术（扫描探针纳米断层扫描）的结合非常适合表征软质多组分纳米结构化材料，如聚合物基质以及掺杂不同类型纳米颗粒（磁性纳米颗粒、量子点、纳米管等）的微结构，还有生物材料。该技术的一个严重问题是缺乏化学和光学表征工具，而使用光学显微光谱可以解决这个问题。在此，我们报告了一种仪器方法的开发，该方法将共聚焦显微光谱和三维扫描探针纳米断层扫描集成在一台仪器中。这种方法保留了SPM和立式光学显微光谱的所有优点，并允许使用这两种技术进行三维多参数表征。作为对所开发系统的首次测试，我们对掺杂了荧光染料和磁性纳米颗粒的荧光磁性微球的形态、磁性和荧光特性进行了相关表征。这项研究的结果可用于为大多数高分辨率近场扫描探针显微镜（扫描近场光学显微镜、针尖增强拉曼光谱、原子力显微镜红外光谱等）获取样品的三维体积图像。这种方法将促成独特技术的发展，这些技术结合了SPM（纳米级形态和广泛的物理参数）和高分辨率光学显微光谱（纳米级化学映射和光学性质）的优点，并允许同时进行三维测量。
