Department of Chemistry, Umeå University, SE901 87 Umeå, Sweden.
Department of Chemistry, Umeå University, SE901 87 Umeå, Sweden.
Bioelectrochemistry. 2017 Dec;118:8-13. doi: 10.1016/j.bioelechem.2017.06.010. Epub 2017 Jun 22.
A supported liquid crystalline cubic phase housing glucose oxidase on an electrode surface has been suggested as bio-anode in a biofuel. The purpose of this investigation is to clarify some aspect on the mediated enzymatic oxidation of glucose in such a bio-anode where the mediator ferrocene-carboxylic acid and glucose were dissolved in the solution. The enzyme glucose oxidase was housed in the water channels of the mono-olein cubic phase. The system was investigated with cyclic voltammetry at different scan rates and the temperature was varied between 15°C and 30°C. The diffusion coefficient of the mediator and also the film resistance was estimated showing a large decrease in the mass-transport properties as the temperature was decreased. The current from mediated oxidation of glucose at the electrode surface increased with decreasing film thickness. The transport of the mediator in the cubic phase was the rate-limiting step in the overall reaction, where the oxidation of glucose took place at the outer surface of the cubic phase.
已提出在生物燃料中，将葡萄糖氧化酶固定在电极表面的支撑液晶立方相作为生物阳极。本研究的目的是阐明在这种生物阳极中酶促葡萄糖氧化的一些方面，其中介体二茂铁羧酸和葡萄糖溶解在溶液中。葡萄糖氧化酶被安置在单油酸立方相的水通道中。该系统在不同扫描速率下通过循环伏安法进行了研究，温度在 15°C 和 30°C 之间变化。介体的扩散系数和薄膜电阻也进行了估算，结果表明随着温度的降低，传质性能大大降低。电极表面介导的葡萄糖氧化产生的电流随着薄膜厚度的减小而增加。在整个反应中，立方相向电极表面的介体传输是限速步骤，其中葡萄糖在外表面发生氧化。
