Bayer M E, Bayer M H
J Virol. 1986 Jan;57(1):258-66. doi: 10.1128/JVI.57.1.258-266.1986.
Phage fd-infected host bacteria revealed three characteristic changes in their envelope. (i) The preferred cleavage plane during freeze-fracturing shifted from the inner to the outer membrane (OM). (ii) The total lipids of the OM of the infected cells increased by 25% without major alterations in the relative concentration of phospholipids. We propose that such an increase would to some extent contribute to the change in the freeze-fracture behavior of the OM; however, additional factors will have to play a role in the apparent fracture resistance of the inner membrane. (iii) Ultrathin sectioning and immunolabeling methods revealed that extrusion of fd phages takes place at membrane adhesion sites of the infected cells.
被噬菌体fd感染的宿主细菌在其包膜上呈现出三个特征性变化。(i) 冷冻断裂过程中的首选裂解平面从内膜转移到外膜（OM）。(ii) 受感染细胞外膜的总脂质增加了25%，而磷脂的相对浓度没有重大变化。我们认为这种增加在一定程度上会导致外膜冷冻断裂行为的改变；然而，其他因素必定在细胞膜明显的抗断裂性中发挥作用。(iii) 超薄切片和免疫标记方法显示，fd噬菌体的挤出发生在受感染细胞的膜粘附部位。
