Grant R A, Webster R E
Virology. 1984 Mar;133(2):315-28. doi: 10.1016/0042-6822(84)90398-2.
The gene V protein/phage single-stranded (SS) DNA complex is an intermediate in the assembly of the filamentous bacteriophage f1. The minor protein content of this complex isolated from wild-type and amber mutant phage-infected Escherichia coli bacteria has been analyzed. Other than the gene V protein, none of the proteins found in purified samples of the complex correspond to any known phage gene products. In particular, the minor coat proteins found in the mature phage particle do not appear to be components of the cytoplasmic gene V protein/f1 SS DNA complex. However, approximately 1-3 molecules of E. coli single-stranded DNA binding protein (SSB) copurify with the complex and may be stably associated with this structure in vivo.
基因V蛋白/噬菌体单链（SS）DNA复合物是丝状噬菌体f1组装过程中的一个中间体。已对从野生型和琥珀突变体噬菌体感染的大肠杆菌中分离出的该复合物的次要蛋白质成分进行了分析。除了基因V蛋白外，在该复合物的纯化样品中发现的蛋白质均与任何已知的噬菌体基因产物不对应。特别是，在成熟噬菌体颗粒中发现的次要衣壳蛋白似乎不是细胞质基因V蛋白/f1单链DNA复合物的组成成分。然而，大约1 - 3个大肠杆菌单链DNA结合蛋白（SSB）分子与该复合物共纯化，并且在体内可能与该结构稳定结合。
