UVA, UVB Light Doses and Harvesting Time Differentially Tailor Glucosinolate and Phenolic Profiles in Broccoli Sprouts.

Broccoli sprouts contain health-promoting glucosinolate and phenolic compounds that can be enhanced by applying ultraviolet light (UV). Here, the effect of UVA or UVB radiation on glucosinolate and phenolic profiles was assessed in broccoli sprouts. Sprouts were exposed for 120 min to low intensity and high intensity UVA (UVA, UVA) or UVB (UVB, UVB) with UV intensity values of 3.16, 4.05, 2.28 and 3.34 W/m², respectively. Harvest occurred 2 or 24 h post-treatment; and methanol/water or ethanol/water (70%, /) extracts were prepared. Seven glucosinolates and 22 phenolics were identified. Ethanol extracts showed higher levels of certain glucosinolates such as glucoraphanin, whereas methanol extracts showed slight higher levels of phenolics. The highest glucosinolate accumulation occurred 24 h after UVB treatment, increasing 4-methoxy-glucobrassicin, glucobrassicin and glucoraphanin by 170, 78 and 73%, respectively. Furthermore, UVA radiation and harvest 2 h afterwards accumulated gallic acid hexoside I (14%), 4--caffeoylquinic acid (42%), gallic acid derivative (48%) and 1-sinapoyl-2,2-diferulolyl-gentiobiose (61%). Increases in sinapoyl malate (12%), gallotannic acid (48%) and 5-sinapoyl-quinic acid (121%) were observed with UVB Results indicate that UV-irradiated broccoli sprouts could be exploited as a functional food for fresh consumption or as a source of bioactive phytochemicals with potential industrial applications.