Kushad M M, Brown A F, Kurilich A C, Juvik J A, Klein B P, Wallig M A, Jeffery E H
Departments of Natural Resources and Environmental Sciences, University of Illinois, Urbana 61801, USA. 61801, USA.
J Agric Food Chem. 1999 Apr;47(4):1541-8. doi: 10.1021/jf980985s.
Glucosinolates were evaluated in 5 groups and 65 accessions of Brassica oleracea (50 broccoli, 4 Brussels sprouts, 6 cabbage, 3 cauliflower, and 2 kale) grown under uniform cultural conditions. Glucosinolates and their concentrations varied among the different groups and within each group. The predominant glucosinolates in broccoli were 4-methylsulfinylbutyl glucosinolate (glucoraphanin), 3-butenyl glucosinolate (gluconapin), and 3-indolylmethyl glucosinoate (glucobrassicin). Glucoraphanin concentration in broccoli ranged from 0.8 micromol g(-1) DW in EV6-1 to 21.7 micromol g(-1) DW in Brigadier. Concentrations of the other glucosinolates in broccoli varied similarly over a wide range. In Brussels sprouts, cabbage, cauliflower, and kale, the predominant glucosinolates were sinigrin (8.9, 7.8, 9.3, and 10.4 micromol g(-1) DW, respectively) and glucobrassicin (3.2, 0.9, 1.3, and 1.2 micromol g(-1) DW, respectively). Brussels sprouts also had significant amounts of gluconapin (6.9 micromol g(-1) DW). Wide variations in glucosinolate content among genotypes suggest differences in their health-promoting properties and the opportunity for enhancement of their levels through genetic manipulation.
在统一栽培条件下种植的5组65份甘蓝（50份西兰花、4份抱子甘蓝、6份卷心菜、3份花椰菜和2份羽衣甘蓝）中对硫代葡萄糖苷进行了评估。硫代葡萄糖苷及其浓度在不同组之间以及每组内部均有所不同。西兰花中主要的硫代葡萄糖苷是4-甲亚磺酰基丁基硫代葡萄糖苷（萝卜硫苷）、3-丁烯基硫代葡萄糖苷（新萝卜硫素）和3-吲哚甲基硫代葡萄糖苷（吲哚-3-甲醇苷）。西兰花中萝卜硫苷的浓度范围为EV6-1中的0.8微摩尔/克干重至准将品种中的21.7微摩尔/克干重。西兰花中其他硫代葡萄糖苷的浓度也在很宽的范围内有类似变化。在抱子甘蓝、卷心菜、花椰菜和羽衣甘蓝中，主要的硫代葡萄糖苷分别是黑芥子硫苷酸钾（分别为8.9、7.8、9.3和10.4微摩尔/克干重）和吲哚-3-甲醇苷（分别为3.2、0.9、1.3和1.2微摩尔/克干重）。抱子甘蓝中也含有大量的新萝卜硫素（6.9微摩尔/克干重）。硫代葡萄糖苷含量在基因型间的广泛差异表明它们在促进健康特性方面存在差异，以及通过基因操作提高其含量的可能性。
