Cicuttin Gabriel L, De Salvo María N, La Rosa Isabel, Dohmen Federico E Gury
Instituto de Zoonosis Luis Pasteur, Av. Díaz Vélez 4821, Buenos Aires, C1405DCD, Argentina.
Instituto de Zoonosis Luis Pasteur, Av. Díaz Vélez 4821, Buenos Aires, C1405DCD, Argentina.
Comp Immunol Microbiol Infect Dis. 2017 Jun;52:1-5. doi: 10.1016/j.cimid.2017.04.004. Epub 2017 May 4.
Bats are potential reservoirs of many vector-borne bacterial pathogens. The aim of the present study was to detect species of Anaplasma, Ehrlichia, Neorickettsia, Rickettsia, Borrelia and Bartonella in Brazilian free-tailed bats (Tadarida brasiliensis, Molossidae) from Buenos Aires city, Argentina. Between 2012 and 2013, 61 T. brasiliensis from urban areas of Buenos Aires city were studied. The samples were molecularly screened by PCR and sequencing. Five bats (8.2%) were positive to Neorickettsia risticii, one (1.6%) was positive to Rickettsia sp. and three bats (4.9%) to Bartonella sp. For molecular characterization, the positive samples were subjected to amplification and sequencing of a fragment of p51 gene for N. risticii, a fragment of citrate synthase gene (gltA) for Rickettsia genus and a fragment of gltA for Bartonella genus. Phylogenetic tree was constructed using the maximum-likelihood method. Phylogenetic analysis of N. risticii detect in our study revealed that it relates to findings in the USA West Coast; Rickettsia sp. detected is phylogenetically within R. bellii group, which also includes many other Rickettsia endosymbionts of insects; and Bartonella sp. found is related to various Bartonella spp. described in Vespertilionidae bats, which are phylogenetically related to Molossidae. Our results are in accordance to previous findings, which demonstrate that insectivorous bats could be infected with vector-borne bacteria representing a potential risk to public health. Future research is necessary to clarify the circulation of these pathogens in bats from Buenos Aires.
蝙蝠是许多媒介传播细菌病原体的潜在宿主。本研究的目的是检测来自阿根廷布宜诺斯艾利斯市的巴西无尾蝠（巴西无尾蝠属，犬吻蝠科）中的无形体属、埃立克体属、新立克次体属、立克次体属、疏螺旋体属和巴尔通体属的种类。在2012年至2013年期间，对来自布宜诺斯艾利斯市市区的61只巴西无尾蝠进行了研究。通过聚合酶链反应（PCR）和测序对样本进行分子筛查。五只蝙蝠（8.2%）对里氏新立克次体呈阳性，一只（1.6%）对立克次体属呈阳性，三只蝙蝠（4.9%）对巴尔通体属呈阳性。为了进行分子特征分析，对阳性样本进行了里氏新立克次体p51基因片段、立克次体属柠檬酸合酶基因（gltA）片段和巴尔通体属gltA片段的扩增和测序。使用最大似然法构建系统发育树。对我们研究中检测到的里氏新立克次体的系统发育分析表明，它与美国西海岸的研究结果相关；检测到的立克次体属在贝氏立克次体组内，该组还包括许多其他昆虫的立克次体内共生菌；发现的巴尔通体属与在蝙蝠科蝙蝠中描述的各种巴尔通体属物种相关，这些物种在系统发育上与犬吻蝠科相关。我们的结果与先前的研究结果一致，先前的研究结果表明食虫蝙蝠可能感染媒介传播细菌，对公共卫生构成潜在风险。有必要进行进一步的研究以阐明这些病原体在布宜诺斯艾利斯蝙蝠中的传播情况。
