Aktas Munir, Ozubek Sezayi
Department of Parasitology, College of Veterinary Medicine, Firat University, 23119, Elazig, Turkey.
Department of Parasitology, College of Veterinary Medicine, Firat University, 23119, Elazig, Turkey.
Comp Immunol Microbiol Infect Dis. 2017 Jun;52:36-42. doi: 10.1016/j.cimid.2017.05.007. Epub 2017 May 26.
Canine tick-borne apicomplexan parasites have emerged in recent years, showing a wider geographic distribution and increased global prevalence. A reverse line blot assay was performed on 219 blood samples collected from domestic dogs for simultaneous detection of all named canine piroplasm species as well as Hepatozoon canis. Ten samples hybridized to the Theileria/Babesia and Babesia catch all probes but did not hybridize to any species-specific probe tested, suggesting the presence of an unrecognized Babesia species or genotype. Sequencing results showed 91.5%, 91.9%, 92.4%, 92.4%, and 89.2% similarity to B. canis, B. vogeli, B. rossi, B. gibsoni, and B. conradae, respectively. The highest homology (98.1-98.5%) observed was with unnamed Babesia sp. isolates (Ludhiana and Malbazar) described in dogs, Babesia sp. of buffalo origin, Babesia sp. Kashi 2, and Babesia orientalis, along with Babesia occultans of cattle origin. The partial cox1 sequence indicated that this isolate was most similar to Babesia sp. 1 HG-2012, with an identity of 86.5%. The survey revealed high prevalence of haemoprotozoans in domestic dogs (57.5%, CI 50.7-64.2), with Hepatozoon canis the most prevalent (54.3%, CI 47.5-61.117%), followed by Babesia sp. (4.6%, CI 2.2-8.2), B. vogeli (1.4%; CI 0.3-3.9), and B. canis (0.4%, CI 0-2.5). Combined infection of Hepatozoon canis and Babesia sp. was detected in five (2.3%, CI 0.7-5.2) samples and of H. canis and B. vogeli in two (0.9%, CI 0.1-3.2) dogs. The study contributes insight into the distribution and phylogenetic diversity of canine piroplasms in Turkey.
近年来，犬蜱传播的顶复门寄生虫不断出现，其地理分布范围更广，全球流行率也有所上升。对从家犬采集的219份血样进行了反向线印迹分析，以同时检测所有已命名的犬梨形虫物种以及犬肝簇虫。有10份样本与泰勒虫属/巴贝斯虫属及巴贝斯虫属通用探针杂交，但未与任何测试的物种特异性探针杂交，这表明存在一种未被识别的巴贝斯虫物种或基因型。测序结果显示，其与犬巴贝斯虫、韦氏巴贝斯虫、罗氏巴贝斯虫、吉氏巴贝斯虫和康氏巴贝斯虫的相似性分别为91.5%、91.9%、92.4%、92.4%和89.2%。观察到的最高同源性（98.1 - 98.5%）是与犬类中描述的未命名巴贝斯虫属分离株（卢迪亚纳和马尔巴扎尔）、水牛源巴贝斯虫属、喀什2号巴贝斯虫属、东方巴贝斯虫以及牛源隐匿巴贝斯虫。部分细胞色素氧化酶亚基1（cox1）序列表明，该分离株与巴贝斯虫属1 HG - 2012最为相似，同一性为86.5%。该调查显示，家犬中血原虫的流行率很高（57.5%，可信区间50.7 - 64.2），其中犬肝簇虫最为流行（54.3%，可信区间47.5 - 61.117%），其次是巴贝斯虫属（4.6%，可信区间2.2 - 8.2）、韦氏巴贝斯虫（1.4%；可信区间0.3 - 3.9）和犬巴贝斯虫（0.4%，可信区间0 - 2.5）。在5份样本（2.3%，可信区间0.7 - 5.2）中检测到犬肝簇虫和巴贝斯虫属的混合感染，在2只犬（0.9%，可信区间0.1 - 3.2）中检测到犬肝簇虫和韦氏巴贝斯虫的混合感染。该研究为深入了解土耳其犬梨形虫的分布和系统发育多样性做出了贡献。
