Zhang Yiming, Wan Dan, Zhou Xihong, Long Ciming, Wu Xin, Li Lan, He Liuqin, Huang Pan, Chen Shuai, Tan Bie, Yin Yulong
Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, Hunan 410125, China; Animal Nutrition and Human Health Laboratory, School of Life Sciences, Hunan Normal University, Changsha, Hunan 410125, China.
Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, Hunan 410125, China.
Biochem Biophys Res Commun. 2017 Sep 2;490(4):1210-1214. doi: 10.1016/j.bbrc.2017.06.187. Epub 2017 Jul 1.
Diurnal variations in serum iron levels have been well documented in clinical studies, and serum iron is an important diagnostic index for iron-deficiency anemia. However, the underlying mechanism of dynamic iron regulation in response to the circadian rhythm is still unclear. In this study, we investigated daily variations in iron status in the plasma and liver of pigs. The transcripts encoding key factors involved in iron uptake and homeostasis were evaluated. The results showed that iron levels in the plasma and liver exhibited diurnal rhythms. Diurnal variations were also observed in transcript levels of divalent metal transporter 1 (DMT1), membrane-associated ferric reductase 1 (DCYTB), and transferrin receptor (TfR) in the duodenum and jejunum, as well as hepcidin (HAMP) and TfR in the liver. Moreover, the results showed a network in which diurnal variations in systemic iron levels were tightly regulated by hepcidin and Tf/TfR via DCYTB and DMT1. These findings provide new insights into circadian iron homeostasis regulation. The diurnal variations in serum iron levels may also have pathophysiological implications for clinical diagnostics related to iron deficiency anemia in pigs.
血清铁水平的昼夜变化在临床研究中已有充分记录，血清铁是缺铁性贫血的重要诊断指标。然而，铁对昼夜节律动态调节的潜在机制仍不清楚。在本研究中，我们调查了猪血浆和肝脏中铁状态的每日变化。评估了编码参与铁摄取和体内平衡的关键因子的转录本。结果表明，血浆和肝脏中的铁水平呈现昼夜节律。十二指肠和空肠中二价金属转运体1（DMT1）、膜相关铁还原酶1（DCYTB）和转铁蛋白受体（TfR）以及肝脏中hepcidin（HAMP）和TfR的转录水平也观察到昼夜变化。此外，结果显示了一个网络，其中全身铁水平的昼夜变化通过DCYTB和DMT1由hepcidin和Tf/TfR紧密调节。这些发现为昼夜铁稳态调节提供了新的见解。血清铁水平的昼夜变化也可能对猪缺铁性贫血的临床诊断具有病理生理学意义。
