Qiu Lingling, Li Zhiteng, Chang Guobin, Bi Yulin, Liu Xiangping, Xu Lu, Zhang Yang, Zhao Wenming, Xu Qi, Chen Guohong
Key Laboratory of Animal Genetics and Breeding, Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou, 225009, PR China.
Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou, Jiangsu 225003, PR China.
Dev Comp Immunol. 2017 Nov;76:292-302. doi: 10.1016/j.dci.2017.06.015. Epub 2017 Jun 30.
Avian leukosis virus subgroup J (ALV-J) is an avian oncogenic retrovirus that has led to severe economic losses in the poultry industry in China in recent decades. Here, using high throughput transcriptome sequencing of HD11 and CEF cells infected with ALV-J, a set of 4804 novel long non-coding transcripts and numerous differentially expressed long non-coding RNAs (lncRNAs) were identified. We also found that they share relatively shorter transcripts and fewer exon numbers compared to mRNA. Correlation analysis suggested that many lncRNAs may activate gene expression in an enhancer-like manner other than through transcriptional regulation. Expression level analyses in vivo showed that three lncRNAs (NONGGAT001975.2, NONGGAT005832.2 and NONGGAT009792.2) may be associated with immune response regulation and could function as novel biomarkers for ALV-J infection. Our findings provides new insight into the pathological process of ALV-J infection and should serve as a high-quality resource for further research on epigenetic influences on disease-resistance breeding as well as immune system and genomic studies.
禽白血病病毒J亚群（ALV-J）是一种禽类致癌逆转录病毒，近几十年来给中国家禽业造成了严重的经济损失。在此，通过对感染ALV-J的HD11和CEF细胞进行高通量转录组测序，鉴定出一组4804个新的长链非编码转录本以及众多差异表达的长链非编码RNA（lncRNA）。我们还发现，与mRNA相比，它们的转录本相对较短，外显子数量较少。相关性分析表明，许多lncRNA可能以类似增强子的方式激活基因表达，而不是通过转录调控。体内表达水平分析表明，三种lncRNA（NONGGAT001975.2、NONGGAT005832.2和NONGGAT009792.2）可能与免疫反应调控有关，并可作为ALV-J感染的新型生物标志物。我们的研究结果为ALV-J感染的病理过程提供了新的见解，应为进一步研究表观遗传对抗病育种以及免疫系统和基因组研究的影响提供高质量的资源。
