Watanabe Junko, Anzawa Kazushi, Mochizuki Takashi
Department of Dermatology, Kanazawa Medical University.
Jpn J Infect Dis. 2017 Sep 25;70(5):544-548. doi: 10.7883/yoken.JJID.2016.424. Epub 2017 Jul 1.
Intraspecies polymorphisms of Microsporum canis strains isolated from human lesions and domesticated cats in Japan were examined by multilocus microsatellite (MS) analysis. Using 9 MS markers, 70 strains were classified into 20 genotypes (genotypes A to T). Of the 9 MS markers used, 5 were considered useful for genotyping, whereas the other 4 were not. The combination of MS2, MS4, and MS7 had the highest resolution power for inter-strain differentiation. Genotype A, present in 15 strains (21.4%), was the most frequent, followed by genotypes H (10 strains, 14.3%), P (8 strains, 11.4%), I (6 strains, 8.6%), and S (6 strains, 8.6%). Eight individual genotypes were present in one strain each. Five episodes of infection of humans and cats living in the same household were analyzed, with strains in all 5 respective households showing the same genotypes. Analysis of geographical distribution established that both genotypes A and H were isolated from 6 prefectures of Honshu and Kyushu islands. To our knowledge, this is the first report showing intraspecies polymorphisms of M. canis isolated in Japan using molecular methods.
通过多位点微卫星（MS）分析，对从日本人类病变和家养猫中分离出的犬小孢子菌菌株的种内多态性进行了研究。使用9个MS标记，将70个菌株分为20个基因型（基因型A至T）。在所使用的9个MS标记中，5个被认为对基因分型有用，而其他4个则不然。MS2、MS4和MS7的组合对菌株间分化具有最高的分辨能力。基因型A存在于15个菌株中（21.4%），是最常见的，其次是基因型H（10个菌株，14.3%）、P（8个菌株，11.4%）、I（6个菌株，8.6%）和S（6个菌株，8.6%）。8个单基因型各存在于一个菌株中。分析了同一家庭中人类和猫的5次感染事件，所有5个家庭中的菌株均显示相同的基因型。地理分布分析表明，基因型A和H均从本州和九州岛的6个县分离得到。据我们所知，这是第一份使用分子方法显示在日本分离出的犬小孢子菌种内多态性的报告。
