Gao Bo, Zhang Jianming, Wang Yuping, Chen Fan, Zheng Chaohui, Xie Lianhui
Fujian Agriculture & Forestry University.
Fujian International Travel Healthcare Center.
Jpn J Infect Dis. 2017 Sep 25;70(5):554-558. doi: 10.7883/yoken.JJID.2016.577. Epub 2017 Jul 1.
Over the past decade, indigenous dengue outbreaks have occurred occasionally in Fujian province in southeastern China because of sporadic imported dengue viruses (DENV). In this study, 3 DENV-2 and 2 DENV-4 strains were isolated from suspected febrile travelers at 2 ports of entry in Fujian between 2013-2015. Complete viral genome sequences of these new isolates were obtained with Sanger chemistry. Genomic sequence analyses revealed that these strains belonged to genotypes of 2-Cosmopolitan and 4-II. Consistent with the patients' travel information, phylogenetic analyses of the complete coding regions also indicated that most of the new isolates were genetically similar to the circulating strains in Southeast Asia rather than previous Chinese strains that were available. Therefore, phylogenetic analyses of the imported DENV demonstrated that multiple introductions of DENV emerged continuously in Fujian, and highlighted the importance of dengue surveillance at entry-exit ports in the subtropical regions of southern China.
在过去十年间，由于偶发的登革病毒（DENV）输入，中国东南部福建省偶尔会发生本土登革热疫情。在本研究中，2013年至2015年间从福建省两个入境口岸的疑似发热旅行者中分离出3株DENV-2和2株DENV-4毒株。采用桑格测序法获得了这些新分离株的完整病毒基因组序列。基因组序列分析表明，这些毒株属于2型泛在基因型和4型II基因型。与患者的旅行信息一致，对完整编码区的系统发育分析也表明，大多数新分离株在基因上与东南亚的流行毒株相似，而非与之前中国已有的毒株相似。因此，对输入性DENV的系统发育分析表明，DENV在福建不断出现多次传入情况，并凸显了中国南方亚热带地区出入境口岸登革热监测的重要性。
