生长激素释放因子（GRF）可提高垂体中游离花生四烯酸水平：脂氧合酶产物的作用。

Growth hormone releasing factor (GRF) increases free arachidonate levels in the pituitary: a role for lipoxygenase products.

作者信息

Canonico P L, Speciale C, Sortino M A, Cronin M J, MacLeod R M, Scapagnini U

出版信息

Life Sci. 1986 Jan 20;38(3):267-72. doi: 10.1016/0024-3205(86)90312-7.

Abstract

GRF, a specific stimulator of GH release, increased in a concentration- and time-dependent manner pituitary [3H]-arachidonate levels in vitro. This effect was antagonized by 100 nM somatostatin. Exogenous arachidonate also stimulated GH release in vitro. Quinacrine, a phospholipase A2 inhibitor, reduced both basal and GRF-stimulated free arachidonate levels as well as GH release. The cyclooxygenase inhibitor indomethacin was ineffective, while BW755c, which also inhibits the lipoxygenase pathway, produced a further increase in the levels of the fatty acid stimulated by GRF and potently reduced GH release. These results provide additional evidence for the involvement of arachidonate metabolism in the hormone-releasing effect of GRF at the somatotroph.

摘要

生长激素释放因子（GRF）是生长激素释放的一种特异性刺激物，在体外可使垂体[3H] - 花生四烯酸水平以浓度和时间依赖性方式升高。这种效应被100 nM的生长抑素所拮抗。外源性花生四烯酸在体外也能刺激生长激素释放。喹那克林是一种磷脂酶A2抑制剂，可降低基础和GRF刺激的游离花生四烯酸水平以及生长激素释放。环氧合酶抑制剂吲哚美辛无效，而同样抑制脂氧合酶途径的BW755c则使GRF刺激的脂肪酸水平进一步升高，并显著降低生长激素释放。这些结果为花生四烯酸代谢参与GRF在促生长激素细胞上的激素释放作用提供了更多证据。