Álvarez María F, Angarita Myrian, Delgado María C, García Celsa, Jiménez-Gomez José, Gebhardt Christiane, Mosquera Teresa
Facultad de Ciencias Agrarias, Universidad Nacional de ColombiaBogotá, Colombia.
Rice Program International Centre for Tropical Agriculture (CIAT)Cali, Colombia.
Front Plant Sci. 2017 Jun 15;8:1040. doi: 10.3389/fpls.2017.01040. eCollection 2017.
The genetic basis of quantitative disease resistance has been studied in crops for several decades as an alternative to gene mediated resistance. The most important disease in the potato crop is late blight, caused by the oomycete Quantitative disease resistance (QDR), as any other quantitative trait in plants, can be genetically mapped to understand the genetic architecture. Association mapping using DNA-based markers has been implemented in many crops to dissect quantitative traits. We used an association mapping approach with candidate genes to identify the first genes associated with quantitative resistance to late blight in Group Phureja. Twenty-nine candidate genes were selected from a set of genes that were differentially expressed during the resistance response to late blight in tetraploid European potato cultivars. The 29 genes were amplified and sequenced in 104 accessions of Group Phureja from Latin America. We identified 238 SNPs in the selected genes and tested them for association with resistance to late blight. The phenotypic data were obtained under field conditions by determining the area under disease progress curve (AUDPC) in two seasons and in two locations. Two genes were associated with QDR to late blight, a potato homolog of thylakoid lumen 15 kDa protein () and a stem 28 kDa glycoprotein (). : A first association mapping experiment was conducted in Group Phureja germplasm, which identified among 29 candidates two genes associated with quantitative resistance to late blight.
几十年来，人们一直在研究作物中数量抗病性的遗传基础，以此作为基因介导抗性的替代方法。马铃薯作物中最重要的病害是晚疫病，由卵菌引起。与植物中的任何其他数量性状一样，数量抗病性（QDR）可以通过遗传定位来了解其遗传结构。利用基于DNA的标记进行关联作图已在许多作物中实施，以剖析数量性状。我们采用候选基因关联作图方法，在富利亚组中鉴定出与晚疫病数量抗性相关的首批基因。从四倍体欧洲马铃薯品种对晚疫病抗性反应过程中差异表达的一组基因中选择了29个候选基因。对来自拉丁美洲的104份富利亚组材料中的这29个基因进行了扩增和测序。我们在所选基因中鉴定出238个单核苷酸多态性（SNP），并测试它们与晚疫病抗性的关联性。通过在两个季节和两个地点测定病害进展曲线下面积（AUDPC），在田间条件下获得表型数据。有两个基因与晚疫病的数量抗病性相关，一个是类囊体腔15 kDa蛋白（）的马铃薯同源物，另一个是茎28 kDa糖蛋白（）。：在富利亚组种质中进行了首次关联作图实验，在29个候选基因中鉴定出两个与晚疫病数量抗性相关的基因。
