Baldan-Martin Montserrat, de la Cuesta Fernando, Alvarez-Llamas Gloria, Ruiz-Hurtado Gema, Ruilope Luis M, Barderas Maria G
Department of Vascular Physiopathology, Hospital Nacional de Paraplejicos (HNP), SESCAM, Toledo, Spain.
Department of Immunology, IIS-Fundacion Jimenez Diaz, Madrid, Spain.
Methods Mol Biol. 2017;1619:141-149. doi: 10.1007/978-1-4939-7057-5_11.
Extracellular vesicles are membrane vesicles related to cell communication. These vesicles consist of proteins, RNA, and microRNA and are an interesting and important tool to understand the processes taking place in the secreting cell, especially in diseases in which its release is often enhanced. The used of blood extracellular vesicles in cardiovascular disease as a low invasive, easily accessible source of circulating markers could give us important information related to pathological process even more with the use of proteomic analysis. In this chapter, we describe a protocol to isolate and proteomic analyze extracellular vesicles from blood associated with cardiovascular disease.
细胞外囊泡是与细胞通讯相关的膜性囊泡。这些囊泡由蛋白质、RNA和微小RNA组成，是了解分泌细胞中发生的过程的一个有趣且重要的工具，尤其是在其释放通常会增强的疾病中。将血液中的细胞外囊泡用作心血管疾病中循环标志物的低侵入性、易于获取的来源，甚至通过蛋白质组学分析，可能会为我们提供与病理过程相关的重要信息。在本章中，我们描述了一种从与心血管疾病相关的血液中分离细胞外囊泡并进行蛋白质组学分析的方案。
