Lee Jaewook, Kim Si-Hyun, Choi Dong-Sic, Lee Jong Seok, Kim Dae-Kyum, Go Gyeongyun, Park Seon-Min, Kim Si Hyun, Shin Jeong Hwan, Chang Chulhun L, Gho Yong Song
Department of Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea.
Section of Microbiology, International Tuberculosis Research Center, Changwon, Republic of Korea.
Proteomics. 2015 Oct;15(19):3331-7. doi: 10.1002/pmic.201500037. Epub 2015 Aug 19.
The release of extracellular vesicles, also known as outer membrane vesicles, membrane vesicles, exosomes, and microvesicles, is an evolutionarily conserved phenomenon from bacteria to eukaryotes. It has been reported that Mycobacterium tuberculosis releases extracellular vesicles harboring immunologically active molecules, and these extracellular vesicles have been suggested to be applicable in vaccine development and biomarker discovery. However, the comprehensive proteomic analysis has not been performed for M. tuberculosis extracellular vesicles. In this study, we identified a total of 287 vesicular proteins by four LC-MS/MS analyses with high confidence. In addition, we identified several vesicular proteins associated with the virulence of M. tuberculosis. This comprehensive proteome profile will help elucidate the pathogenic mechanism of M. tuberculosis. The data have been deposited to the ProteomeXchange with identifier PXD001160 (http://proteomecentral.proteomexchange.org/dataset/PXD001160).
细胞外囊泡的释放，也被称为外膜囊泡、膜囊泡、外泌体和微囊泡，是一种从细菌到真核生物都存在的进化保守现象。据报道，结核分枝杆菌会释放携带免疫活性分子的细胞外囊泡，并且这些细胞外囊泡已被认为可应用于疫苗开发和生物标志物发现。然而，尚未对结核分枝杆菌细胞外囊泡进行全面的蛋白质组学分析。在本研究中，我们通过四次高可信度的液相色谱-串联质谱分析共鉴定出287种囊泡蛋白。此外，我们还鉴定出了几种与结核分枝杆菌毒力相关的囊泡蛋白。这种全面的蛋白质组图谱将有助于阐明结核分枝杆菌的致病机制。数据已提交至蛋白质组交换库，标识符为PXD001160（http://proteomecentral.proteomexchange.org/dataset/PXD001160）。
