Christinat Nicolas, Morin-Rivron Delphine, Masoodi Mojgan
Lipid Biology, Nestlé Institute of Health Sciences, EPFL Innovation Park, bâtiment H, Lausanne, 1015, Switzerland.
Faculty of Medicine, Department of Nutritional Sciences, University of Toronto, Toronto, ON, Canada.
Methods Mol Biol. 2017;1619:183-191. doi: 10.1007/978-1-4939-7057-5_14.
Nonesterified fatty acids are important biological molecules which have multiple functions such as energy storage, gene regulation, or cell signaling. Comprehensive profiling of nonesterified fatty acids in biofluids can facilitate studying and understanding their roles in biological systems. For these reasons, we have developed and validated a high-throughput, nontargeted lipidomics method coupling liquid chromatography to high-resolution mass spectrometry for quantitative analysis of nonesterified fatty acids. Sufficient chromatographic separation is achieved to separate positional isomers such as polyunsaturated and branched-chain species and quantify a wide range of nonesterified fatty acids in human plasma samples. However, this method is not limited only to these fatty acid species and offers the possibility to perform untargeted screening of additional nonesterified fatty acid species.
非酯化脂肪酸是重要的生物分子，具有多种功能，如能量储存、基因调控或细胞信号传导。对生物流体中的非酯化脂肪酸进行全面分析有助于研究和理解它们在生物系统中的作用。基于这些原因，我们开发并验证了一种高通量、非靶向脂质组学方法，该方法将液相色谱与高分辨率质谱联用，用于非酯化脂肪酸的定量分析。该方法能够实现充分的色谱分离，以分离多不饱和脂肪酸和支链脂肪酸等位置异构体，并对人血浆样本中的多种非酯化脂肪酸进行定量。然而，该方法不仅限于这些脂肪酸种类，还提供了对其他非酯化脂肪酸种类进行非靶向筛选的可能性。
