采用液相色谱-高分辨率质谱法（LC-HRMS）对健康受试者和糖尿病患者血浆中的游离脂肪酸进行脂质组学分析。

Lipidomics Analysis of Free Fatty Acids in Human Plasma of Healthy and Diabetic Subjects by Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS).

作者信息

Kokotou Maroula G, Mantzourani Christiana, Batsika Charikleia S, Mountanea Olga G, Eleftheriadou Ioanna, Kosta Ourania, Tentolouris Nikolaos, Kokotos George

机构信息

Department of Chemistry, National and Kapodistrian University of Athens, 15771 Athens, Greece.

Laboratory of Chemistry, Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece.

出版信息

Biomedicines. 2022 May 20;10(5):1189. doi: 10.3390/biomedicines10051189.

Targeted analytical methods for the determination of free fatty acids (FFAs) in human plasma are of high interest because they may help in identifying biomarkers for diseases and in monitoring the progress of a disease. The determination of FFAs is of particular importance in the case of metabolic disorders because FFAs have been associated with diabetes. We present a liquid chromatography-high resolution mass spectrometry (LC-HRMS) method, which allows the simultaneous determination of 74 FFAs in human plasma. The method is fast (10-min run) and straightforward, avoiding any derivatization step and tedious sample preparation. A total of 35 standard saturated and unsaturated FFAs, as well as 39 oxygenated (either hydroxy or oxo) saturated FFAs, were simultaneously detected and quantified in plasma samples from 29 subjects with type 2 diabetes mellitus (T2D), 14 with type 1 diabetes mellitus (T1D), and 28 healthy subjects. Alterations in the levels of medium-chain FFAs (C6:0 to C10:0) were observed between the control group and T2D and T1D patients.

用于测定人血浆中游离脂肪酸（FFA）的靶向分析方法备受关注，因为它们可能有助于识别疾病的生物标志物并监测疾病进展。在代谢紊乱的情况下，FFA的测定尤为重要，因为FFA与糖尿病有关。我们提出了一种液相色谱-高分辨率质谱（LC-HRMS）方法，该方法能够同时测定人血浆中的74种FFA。该方法快速（运行时间为10分钟）且操作简便，无需任何衍生化步骤和繁琐的样品制备。在29例2型糖尿病（T2D）患者、14例1型糖尿病（T1D）患者和28例健康受试者的血浆样本中，同时检测并定量了总共35种标准饱和和不饱和FFA，以及39种氧化（羟基或氧代）饱和FFA。在对照组与T2D和T1D患者之间观察到中链FFA（C6:0至C10:0）水平的变化。